Research ArticleResearch Article

Resveratrol Induces Apoptosis in Human Osteosarcoma MG63 Cells

Yan Liu, Xin Wang, Yuxin Xie, Jingui Zhang, Qingshan Wang and Xianhui Xu
Chinese Journal of Clinical Oncology October 2008, 5 (5) 361-366; DOI: https://doi.org/10.1007/s11805-008-0361-3

Article Figures & Data

Figures

  • Fig.1.
    Fig.1. Morphological observation by transmission electron microscopy in MG63 cells treated with 20 mg/L of resveratrol for 72 h.

    MG63 cells showed apoptotic morphological changes of the chromatin such as being condensed and marginated (as showed by arrows in C ); There were lots of vacuolus in the cytoplasm (as shown by arrows in D). A, control cells × 4,000; B, control cells × 15,000; C, treated with resveratrol × 4,000; D, treated with resveratrol × 15,000.

  • Fig.2.
    Fig.2. Effect of resveratrol on growth inhibition in MG63 cell.

    Embedded Image; n = 3; *, P < 0.05, **, P < 0.01; compared with control group.

  • Fig.3.
    Fig.3. Apoptotic rate of MG63 detected by flow cytometry.

    A, control cells; B&C, cells treated with resveratrol (10 or 20 mg/L) for 72 h, respectively. A sub-diploid peak was found before the G0/G1trasition, which was a typical apoptotic cell peak.

  • Fig.4.
    Fig.4. Analysis of the p21cip1/WAF1 and survivin mRNA expression in MG63 cells after treatmeat with resveratrol for 72 h.

    A and B show the mRNA expression of p21cip1/WAF1 and survivin respectively. Lane M, DNA marker; Lane 1, control cells; Lane 2, 10 mg/L resveratrol; Lane 3, 20 mg/L resveratrol. C shows the average of semi-quantitative amounts of p21cip1/WAF1 and survivin mRNA respectively. mRNA expression is presented as a ratio to internal standard. x ± s, n = 3, *P < 0.05, **P < 0.01, compared with control cells.

Tables

  • Table 1.

    Effect of resveratrol on cell cycle parameters and apoptosis.

    Concentration
    (mg/L)    		Apoptosis rates
    (%)    		Cell cycle (%)
    G0/G1SG2/M
    04.85 ± 0.2170.7 ± 1.7020.8 ± 1.238.5 ± 0.69
    1011.90 ± 0.63**75.5 ± 1.25*16.5 ± 0.817.0 ± 0.84
    2019.74 ± 0.88**83.4 ± 1.21*10.7 ± 0.984.6 ± 1.03

    • Embedded Image; n = 3;

    • ↵* P < 0.05;

    • ↵** P < 0.01; compared with control group.

  • Table 2.

    Effects of resveratrol on the expression of p21cip1/WAF1 and survivin.

    Concentration (mg/L)Fluorescence index
    Survivinp21cip1/WAF1
    01.00 ± 0.021.00 ± 0.01
    100.94 ± 0.041.09 ± 0.03*
    200.90 ± 0.06*1.74 ± 0.06**

    • Embedded Image; n = 3;

    • ↵* P < 0.05;

    • ↵** P < 0.01; compared with control group.

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