RT Journal Article
SR Electronic
T1 Resveratrol Induces Apoptosis in Human Osteosarcoma MG63 Cells
JF Chinese Journal of Clinical Oncology
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 361
OP 366
DO 10.1007/s11805-008-0361-3
VO 5
IS 5
A1 Yan Liu
A1 Xin Wang
A1 Yuxin Xie
A1 Jingui Zhang
A1 Qingshan Wang
A1 Xianhui Xu
YR 2008
UL http://www.cancerbiomed.org/content/5/5/361.abstract
AB OBJECTIVE To investigate apoptosis in human osteosarcoma MG63 cells induced by resveratrol and the molecular mechanism involved.METHODS MG63 cells were treated with different concentrations of resveratrol and transmission electron microscopy was used to observe morphological changes occurring in apoptosis. The MTT method was used to determine the inhibitory rate and flow cytometry was used to assess apoptosis and to analyze the expression of the p21cip1/WAF1 and survivin proteins; the expression of p21cip1/WAF1 and survivin mRNAs was analyzed by the reverse transcriptase polymerase chain reaction (RT-PCR).RESULTS After resveratrol treatment, the growth of the MG63 cells was significantly inhibited in a time- and dose-dependent fashion. By transmission electron microscopy, the cells displayed morphological changes characteristic of apoptosis, including formation of cytoplasmic vacuoles, chromatin condensation and margination. Flow cytometry showed that the growth of the cells was inhibited after resveratrol (10 mg/L and 20 mg/L) treatment. The inhibitory rates were (11.9 ± 0.63)% and (19.7 ± 0.88)% respectively. The quantity of treated cells in G0/G1 transition was increased, but the number in the S phase and G2/M transition was decreased. A subdiploid peak was observed. The expression of p21cip1/WAF1 was up-regulated while survivin was down-regulated.CONCLUSION Resveratrol can inhibit growth and induce apoptosis of MG63 cells. Its molecular mechanism might be related to modulation of survivin and p21cip1/WAF1 expression.