Research ArticleResearch Article

Inhibition of Differentiation by Transforming Growth Factor ß1 in Rhabdomyosarcoma Cells

Shouli Wang, Huihua Yao, Zhenghong Qin, Shigang Li, Yizhong Feng, Xiuzhen Wang, Min Deng, Lingling Guo and Lifeng Zhang
Chinese Journal of Clinical Oncology October 2007, 4 (5) 327-332; DOI: https://doi.org/10.1007/s11805-007-0327-x

Article Figures & Data

Figures

  • Fig.1.
    Fig.1.

    Concentration-response to TGF-ß1 on growth of RD cells. RD cells were treated with TGF-ß1 (0.1, 0.2, 0.5, 1, 2, and 5 ng/ml) or without TGF-ß1 for 1~6 d. Cell viability was quantified by the MTT assay. Each experiment was performed three times.

  • Fig.2.
    Fig.2.

    MTT analysis of the effect of TGF-ß1 (0.1 ng/ml) on the cell viability in the presence of 9CRA. 9CRA suppressed growth of RD cells (P<0.001), whereas, there was no significant difference between control and 9CRA+TGF-ß1 (0.1 ng/ml) groups throughout the 6-day time period.

  • Fig.3.
    Fig.3.

    Inhibition of myogenic differentiation by TGF-ß1. Immunofluorescence analysis of RD cells in control (ethanol) (A, D, G, and J×600), in DMEM containing 5 uM 9CRA without TGF-ß1 (B, E, H, and K) or with 5 uM 9CRA plus TGF-ß1 (0.1 ng/ml) (C, F, I, and L×600) for 6 days. Cells were stained with anti-sarcomeric actin (A, B, and C×600), anti-MyHC (D, E, and F×600), anti-myogenin (G, H, and I×600), and anti-MyoD1 (J, K, and L×600). Positive cells were detected and quantified as described in materials and methods.

Tables

  • Table 1.

    Analysis of myogenic differentiation.

    ProteinsControlPlus 9CRAPlus 9CRA and 0.1 ng/ml TGF-ß1
    Actin203622
    MyHC103118
    Myogenin5104
    MyoD1888

    • Values represent percent positive cells

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