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Review ArticleReview

Changing paradigm of cancer therapy: precision medicine by next-generation sequencing

Yuan Xue and William R. Wilcox
Cancer Biology & Medicine March 2016, 13 (1) 12-18; DOI: https://doi.org/10.28092/j.issn.2095-3941.2016.0003
Yuan Xue
1Fulgent Diagnostics, Temple City, CA 91780, USA
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William R. Wilcox
2Department of Human Genetics, Emory University, Atlanta, GA 30322, USA
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    How NGS technology enables precision medicine in cancer.

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    Table1

    Examples of commercially available cancer panels

    CompanyPanel nameNumber of genesCoverageNote
    Life sciencesIon ampliSeq hotspot cancer panel50None indicatedCompatible with FFPE sample
    Life sciencesIon ampliSeq comprehensive cancer panel409None indicatedDetect CNV; compatible with FFPE sample
    IlluminaTruSight cancer panel9420×Germline only
    IlluminaTruSight tumor 15 panel15≥500×Solid tumor somatic variants
    IlluminaTruSight amplicon cancer panel48~1000×Somatic mutational hotspots
    IlluminaTruSight RNA pan-cancer panel21,043 exonic region3 million reads per sampleGene expression, variant and fusion detection in 1,385 cancer associated genes
    IDTxGen® pan-cancer panel127>97% of targeted covered at 30× Can be expanded by adding custom probes
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Cancer Biology and Medicine: 13 (1)
Cancer Biology & Medicine
Vol. 13, Issue 1
1 Mar 2016
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Changing paradigm of cancer therapy: precision medicine by next-generation sequencing
Yuan Xue, William R. Wilcox
Cancer Biology & Medicine Mar 2016, 13 (1) 12-18; DOI: 10.28092/j.issn.2095-3941.2016.0003

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Changing paradigm of cancer therapy: precision medicine by next-generation sequencing
Yuan Xue, William R. Wilcox
Cancer Biology & Medicine Mar 2016, 13 (1) 12-18; DOI: 10.28092/j.issn.2095-3941.2016.0003
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    • Abstract
    • Introduction
    • Technical perspectives of NGS in solid tumor tissue
    • The impact of NGS on precision cancer therapy
    • Limitations and concerns of NGS
    • Conclusions
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Keywords

  • precision medicine
  • cancer therapy
  • next-generation sequencing
  • solid tumor

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