Skip to main content

Main menu

  • Home
  • About
    • About CBM
    • Editorial Board
    • Announcement
  • Articles
    • Ahead of print
    • Current Issue
    • Archive
    • Collections
    • Cover Story
  • For Authors
    • Instructions for Authors
    • Resources
    • Submit a Manuscript
  • For Reviewers
    • Become a Reviewer
    • Instructions for Reviewers
    • Resources
    • Outstanding Reviewer
  • Subscription
  • Alerts
    • Email Alerts
    • RSS Feeds
    • Table of Contents
  • Contact us
  • Other Publications
    • cbm

User menu

  • My alerts

Search

  • Advanced search
Cancer Biology & Medicine
  • Other Publications
    • cbm
  • My alerts
Cancer Biology & Medicine

Advanced Search

 

  • Home
  • About
    • About CBM
    • Editorial Board
    • Announcement
  • Articles
    • Ahead of print
    • Current Issue
    • Archive
    • Collections
    • Cover Story
  • For Authors
    • Instructions for Authors
    • Resources
    • Submit a Manuscript
  • For Reviewers
    • Become a Reviewer
    • Instructions for Reviewers
    • Resources
    • Outstanding Reviewer
  • Subscription
  • Alerts
    • Email Alerts
    • RSS Feeds
    • Table of Contents
  • Contact us
  • Follow cbm on Twitter
  • Visit cbm on Facebook
Review ArticleReview

Single-cell analyses of circulating tumor cells

Xi-Xi Chen and Fan Bai
Cancer Biology & Medicine September 2015, 12 (3) 184-192; DOI: https://doi.org/10.7497/j.issn.2095-3941.2015.0056
Xi-Xi Chen
1Biodynamic Optical Imaging Center, School of Life Science, Peking University, Beijing 100871, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fan Bai
1Biodynamic Optical Imaging Center, School of Life Science, Peking University, Beijing 100871, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: fbai{at}pku.edu.cn
  • Article
  • Figures & Data
  • Info & Metrics
  • References
  • PDF
Loading

References

  1. 1.↵
    1. Ashworth TR.
    A case of cancer in which cells similar to those in the tumors were seen in the blood after death. Aus Med J 1869;14:146–149.
    OpenUrl
  2. 2.↵
    1. Fidler IJ.
    The pathogenesis of cancer metastasis: the 'seed and soil' hypothesis revisited. Nat Rev Cancer 2003;3:453–458.
    OpenUrlCrossRefPubMedWeb of Science
  3. 3.↵
    1. Thiery JP.
    Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer 2002;2:442–454.
    OpenUrlCrossRefPubMedWeb of Science
  4. 4.
    1. Thiery JP,
    2. Lim CT.
    Tumor dissemination: an EMT affair. Cancer Cell 2013;23:272–273.
    OpenUrlCrossRefPubMed
  5. 5.↵
    1. Kalluri R.
    EMT: when epithelial cells decide to become mesenchymal-like cells. J Clin Invest 2009;119:1417.
    OpenUrlCrossRefPubMedWeb of Science
  6. 6.↵
    1. Tsuji T,
    2. Ibaragi S,
    3. Shima K,
    4. Hu MG,
    5. Katsurano M,
    6. Sasaki A, et al.
    Epithelial-mesenchymal transition induced by growth suppressor p12CDK2-AP1 promotes tumor cell local invasion but suppresses distant colony growth. Cancer Res 2008;68:10377–10386.
    OpenUrlAbstract/FREE Full Text
  7. 7.↵
    1. Kallergi G,
    2. Papadaki MA,
    3. Politaki E,
    4. Mavroudis D,
    5. Georgoulias V,
    6. Agelaki S.
    Epithelial to mesenchymal transition markers expressed in circulating tumour cells of early and metastatic breast cancer patients. Breast Cancer Res 2011;13:R59.
    OpenUrlCrossRefPubMed
  8. 8.↵
    1. Yu M,
    2. Bardia A,
    3. Wittner BS,
    4. Stott SL,
    5. Smas ME,
    6. Ting DT, et al.
    Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science 2013;339:580–584.
    OpenUrlAbstract/FREE Full Text
  9. 9.
    1. Hou JM,
    2. Krebs M,
    3. Ward T,
    4. Sloane R,
    5. Priest L,
    6. Hughes A, et al.
    Circulating tumor cells as a window on metastasis biology in lung cancer. Am J Pathol 2011;178:989–996.
    OpenUrlCrossRefPubMedWeb of Science
  10. 10.
    1. Lecharpentier A,
    2. Vielh P,
    3. Perez-Moreno P,
    4. Planchard D,
    5. Soria J,
    6. Farace F.
    Detection of circulating tumour cells with a hybrid (epithelial/mesenchymal) phenotype in patients with metastatic non-small cell lung cancer. Br J Cancer 2011;105:1338–1341.
    OpenUrlCrossRefPubMedWeb of Science
  11. 11.↵
    1. Balasubramanian P,
    2. Lang JC,
    3. Jatana KR,
    4. Miller B,
    5. Ozer E,
    6. Old M, et al.
    Multiparameter analysis, including EMT markers, on negatively enriched blood samples from patients with squamous cell carcinoma of the head and neck. PLoS One 2012;7:e42048.
  12. 12.↵
    1. Krebs MG,
    2. Metcalf RL,
    3. Carter L,
    4. Brady G,
    5. Blackhall FH,
    6. Dive C.
    Molecular analysis of circulating tumour cells-biology and biomarkers. Nat Rev Clin Oncol 2014;11:129–144.
    OpenUrlCrossRefPubMed
  13. 13.↵
    1. Al-Hajj M,
    2. Wicha MS,
    3. Benito-Hernandez A,
    4. Morrison SJ,
    5. Clarke MF.
    Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci U S A 2003;100:3983–3988.
    OpenUrlAbstract/FREE Full Text
  14. 14.
    1. Ricci-Vitiani L,
    2. Lombardi DG,
    3. Pilozzi E,
    4. Biffoni M,
    5. Todaro M,
    6. Peschle C, et al.
    Identification and expansion of human colon-cancer-initiating cells. Nature 2007;445:111–115.
    OpenUrlCrossRefPubMedWeb of Science
  15. 15.↵
    1. Stewart JM,
    2. Shaw PA,
    3. Gedye C,
    4. Bernardini MQ,
    5. Neel BG,
    6. Ailles LE.
    Phenotypic heterogeneity and instability of human ovarian tumor-initiating cells. Proc Natl Acad Sci U S A 2011;108:6468–6473.
    OpenUrlAbstract/FREE Full Text
  16. 16.↵
    1. Fidler IJ.
    Metastasis: Quantitative Analysis of Distribution and Fate of Tumor Emboli Labeled With 125I-5-Iodo-2'-deoxyuridine2,3. J Natl Cancer Inst 1970;45:773–782.
    OpenUrlPubMedWeb of Science
  17. 17.↵
    1. Fidler IJ.
    The relationship of embolic homogeneity, number, size and viability to the incidence of experimental metastasis. Eur J Cancer 1973;9:223–227.
    OpenUrlCrossRefPubMedWeb of Science
  18. 18.↵
    1. Thompson SC.
    The colony forming efficiency of single cells and cell aggregates from a spontaneous mouse mammary tumour using the lung colony assay. Br J Cancer 1974;30:332.
    OpenUrlPubMed
  19. 19.↵
    1. Duda DG,
    2. Duyverman AM,
    3. Kohno M,
    4. Snuderl M,
    5. Steller EJ,
    6. Fukumura D, et al.
    Malignant cells facilitate lung metastasis by bringing their own soil. Proc Natl Acad Sci U S A 2010;107:21677–21682.
    OpenUrlAbstract/FREE Full Text
  20. 20.↵
    1. Küsters B,
    2. Kats G,
    3. Roodink I,
    4. Verrijp K,
    5. Wesseling P,
    6. Ruiter DJ, et al.
    Micronodular transformation as a novel mechanism of VEGF-A-induced metastasis. Oncogene 2007;26:5808–5815.
    OpenUrlCrossRefPubMed
  21. 21.↵
    1. Yu M,
    2. Stott S,
    3. Toner M,
    4. Maheswaran S,
    5. Haber DA.
    Circulating tumor cells: approaches to isolation and characterization. J Cell Biol 2011;192:373–382.
    OpenUrlAbstract/FREE Full Text
  22. 22.↵
    1. Riethdorf S,
    2. Fritsche H,
    3. Müller V,
    4. Rau T,
    5. Schindlbeck C,
    6. Rack B, et al.
    Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system. Clin Cancer Res 2007;13:920–928.
    OpenUrlAbstract/FREE Full Text
  23. 23.↵
    1. Harb W,
    2. Fan A,
    3. Tran T,
    4. Danila DC,
    5. Keys D,
    6. Schwartz M, et al.
    Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qPCR Assay. Transl Oncol 2013;6:528–538.
    OpenUrlCrossRefPubMed
  24. 24.↵
    1. Thege FI,
    2. Lannin TB,
    3. Saha TN,
    4. Tsai S,
    5. Kochman ML,
    6. Hollingsworth MA, et al.
    Microfluidic immunocapture of circulating pancreatic cells using parallel EpCAM and MUC1 capture: characterization, optimization and downstream analysis. Lab Chip 2014;14:1775–1784.
    OpenUrlPubMed
  25. 25.↵
    1. Saucedo-Zeni N,
    2. Mewes S,
    3. Niestroj R,
    4. Gasiorowski L,
    5. Murawa D,
    6. Nowaczyk P, et al.
    A novel method for the in vivo isolation of circulating tumor cells from peripheral blood of cancer patients using a functionalized and structured medical wire. Int J Oncol 2012;41:1241–1250.
    OpenUrlPubMed
  26. 26.↵
    1. Ozkumur E,
    2. Shah AM,
    3. Ciciliano JC,
    4. Emmink BL,
    5. Miyamoto DT,
    6. Brachtel E, et al.
    Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells. Sci Transl Med 2013;5:179ra47.
    OpenUrlAbstract/FREE Full Text
  27. 27.↵
    1. Vona G,
    2. Sabile A,
    3. Louha M,
    4. Sitruk V,
    5. Romana S,
    6. Schütze K, et al.
    Isolation by Size of Epithelial Tumor Cells. Am J Pathol 2000;156:57–63.
    OpenUrlCrossRefPubMedWeb of Science
  28. 28.↵
    1. Wu S,
    2. Liu Z,
    3. Liu S,
    4. Lin L,
    5. Yang W,
    6. Xu J.
    Enrichment and enumeration of circulating tumor cells by efficient depletion of leukocyte fractions. Clin Chem Lab Med 2014;52:243–251.
    OpenUrlPubMed
  29. 29.↵
    1. Joosse SA,
    2. Gorges TM,
    3. Pantel K.
    Biology, detection, and clinical implications of circulating tumor cells. EMBO Mol Med 2015;7:1–11.
    OpenUrlAbstract/FREE Full Text
  30. 30.↵
    1. Riahi R,
    2. Gogoi P,
    3. Sepehri S,
    4. Zhou Y,
    5. Handique K,
    6. Godsey J, et al.
    A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer. Int J Oncol 2014;44:1870–1878.
    OpenUrlPubMed
  31. 31.↵
    1. Cristofanilli M,
    2. Hayes DF,
    3. Budd GT,
    4. Ellis MJ,
    5. Stopeck A,
    6. Reuben JM, et al.
    Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer. J Clin Oncol 2005;23:1420–1430.
    OpenUrlAbstract/FREE Full Text
  32. 32.
    1. Cohen SJ,
    2. Punt CJ,
    3. Iannotti N,
    4. Saidman BH,
    5. Sabbath KD,
    6. Gabrail NY, et al.
    Relationship of circulating tumor cells to tumor response, progression-free survival, and overall survival in patients with metastatic colorectal cancer. J Clin Oncol 2008;26:3213–3221.
    OpenUrlAbstract/FREE Full Text
  33. 33.↵
    1. de Bono JS,
    2. Scher HI,
    3. Montgomery RB,
    4. Parker C,
    5. Miller MC,
    6. Tissing H, et al.
    Circulating tumor cells predict survival benefit from treatment in metastatic castration-resistant prostate cancer. Clin Cancer Res 2008;14:6302–6309.
    OpenUrlAbstract/FREE Full Text
  34. 34.↵
    1. Krebs MG,
    2. Sloane R,
    3. Priest L,
    4. Lancashire L,
    5. Hou J-M,
    6. Greystoke A, et al.
    Evaluation and prognostic significance of circulating tumor cells in patients with non-small-cell lung cancer. J Clin Oncol 2011;29:1556–1563.
    OpenUrlAbstract/FREE Full Text
  35. 35.↵
    1. Rao C,
    2. Bui T,
    3. Connelly M,
    4. Doyle G,
    5. Karydis I,
    6. Middleton MR, et al.
    Circulating melanoma cells and survival in metastatic melanoma. Int J Oncol 2011;38:755–760.
    OpenUrlPubMed
  36. 36.↵
    1. Nichols AC,
    2. Lowes LE,
    3. Szeto CCT,
    4. Basmaji J,
    5. Dhaliwal S,
    6. Chapeskie C, et al.
    Detection of circulating tumor cells in advanced head and neck cancer using the CellSearch system. Head Neck 2012;34:1440–1444.
    OpenUrlCrossRefPubMed
  37. 37.↵
    1. Han L,
    2. Chen W,
    3. Zhao Q.
    Prognostic value of circulating tumor cells in patients with pancreatic cancer: a meta-analysis. Tumour Biol 2014;35:2473–2480.
    OpenUrlCrossRef
  38. 38.↵
    1. Iinuma H,
    2. Watanabe T,
    3. Mimori K.
    Clinical significance of circulating tumor cells, including cancer stem-like cells, in peripheral blood for recurrence and prognosis in patients with Dukes' stage B and C colorectal cancer. J Clin Oncol 2011;29:1547–1555.
    OpenUrlAbstract/FREE Full Text
  39. 39.↵
    1. Rack B,
    2. Schindlbeck C,
    3. Jückstock J,
    4. Andergassen U,
    5. Hepp P,
    6. Zwingers T, et al.
    Circulating tumor cells predict survival in early average-to-high risk breast cancer patients. J Natl Cancer Inst 2014;106:2504–2511.
    OpenUrl
  40. 40.↵
    1. de Bono JS,
    2. Logothetis CJ,
    3. Molina A,
    4. Fizazi K,
    5. North S,
    6. Chu L, et al.
    Abiraterone and increased survival in metastatic prostate cancer. N Engl J Med 2011;364:1995–2005.
    OpenUrlCrossRefPubMedWeb of Science
  41. 41.↵
    1. Yap TA,
    2. Olmos D,
    3. Brunetto AT,
    4. Tunariu N,
    5. Barriuso J,
    6. Riisnaes R, et al.
    Phase I trial of a selective c-MET inhibitor ARQ197 incorporating proof of mechanism pharmacodynamic studies J Clin Oncol 2011;29:1271–1279.
    OpenUrlAbstract/FREE Full Text
  42. 42.↵
    1. Bianchini D,
    2. Omlin AG,
    3. Pezaro CJ,
    4. Mukherji D,
    5. Lorente Estelles D,
    6. Zivi A, et al.
    First-in-human phase I study of EZN-4176, a locked nucleic acid antisense oligonucleotide (LNA-ASO) to androgen receptor (AR) mRNA in patients with castration-resistant prostate cancer (CRPC). J Clin Oncol 2013;31:abstr 5052.
  43. 43.↵
    1. Attard G,
    2. Swennenhuis JF,
    3. Olmos D,
    4. Reid AH,
    5. Vickers E,
    6. A'Hern R, et al.
    Characterization of ERG, AR and PTEN gene status in circulating tumor cells from patients with castration-resistant prostate cancer. Cancer Res 2009;69:2912–2918.
    OpenUrlAbstract/FREE Full Text
  44. 44.↵
    1. Danila DC,
    2. Anand A,
    3. Sung CC,
    4. Heller G,
    5. Leversha MA,
    6. Cao L, et al.
    TMPRSS2-ERG Status in Circulating Tumor Cells as a Predictive Biomarker of Sensitivity in Castration-Resistant Prostate Cancer Patients Treated With Abiraterone Acetate. Eur Urol 2011;60:897–904.
    OpenUrlCrossRefPubMed
  45. 45.↵
    1. Fehm T,
    2. Müller V,
    3. Aktas B,
    4. Janni W,
    5. Schneeweiss A,
    6. Stickeler E, et al.
    HER2 status of circulating tumor cells in patients with metastatic breast cancer: a prospective, multicenter trial. Breast Cancer Res Treat 2010;124:403–412.
    OpenUrlCrossRefPubMedWeb of Science
  46. 46.↵
    1. Maheswaran S,
    2. Sequist LV,
    3. Nagrath S,
    4. Ulkus L,
    5. Brannigan B,
    6. Collura CV, et al.
    Detection of mutations in EGFR in circulating lung-cancer cells. N Engl J Med 2008;359:366–377.
    OpenUrlCrossRefPubMedWeb of Science
  47. 47.↵
    1. Tang F,
    2. Barbacioru C,
    3. Wang Y,
    4. Nordman E,
    5. Lee C,
    6. Xu N, et al.
    mRNA-Seq whole-transcriptome analysis of a single cell. Nat Methods 2009;6:377–382.
    OpenUrlCrossRefPubMedWeb of Science
  48. 48.↵
    1. Ramsköld D,
    2. Luo S,
    3. Wang YC,
    4. Li R,
    5. Deng Q,
    6. Faridani OR, et al.
    Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells. Nat Biotechnol 2012;30:777–782.
    OpenUrlCrossRefPubMed
  49. 49.↵
    1. Dean FB,
    2. Nelson JR,
    3. Giesler TL,
    4. Lasken RS.
    Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification. Genome Res 2001;11:1095–1099.
    OpenUrlAbstract/FREE Full Text
  50. 50.↵
    1. Dean FB,
    2. Hosono S,
    3. Fang L,
    4. Wu X,
    5. Faruqi AF,
    6. Bray-Ward P, et al.
    Comprehensive human genome amplification using multiple displacement amplification. Proc Natl Acad Sci U S A 2002;99:5261–5266.
    OpenUrlAbstract/FREE Full Text
  51. 51.↵
    1. Zong C,
    2. Lu S,
    3. Chapman AR,
    4. Xie XS.
    Genome-wide detection of single-nucleotide and copy-number variations of a single human cell. Science 2012;338:1622–1626.
    OpenUrlAbstract/FREE Full Text
  52. 52.↵
    1. Ni X,
    2. Zhuo M,
    3. Su Z,
    4. Duan J,
    5. Gao Y,
    6. Wang Z, et al.
    Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients. Proc Natl Acad Sci U S A 2013;110:21083–21088.
    OpenUrlAbstract/FREE Full Text
  53. 53.↵
    1. Heitzer E,
    2. Auer M,
    3. Gasch C,
    4. Pichler M,
    5. Ulz P,
    6. Hoffmann EM, et al.
    Complex tumor genomes inferred from single circulating tumor cells by array-CGH and next-generation sequencing. Cancer Res 2013;73:2965–2975.
    OpenUrlAbstract/FREE Full Text
  54. 54.↵
    1. Steinert G,
    2. Scholch S,
    3. Niemietz T,
    4. Iwata N,
    5. Garcia SA,
    6. Behrens B, et al.
    Immune escape and survival mechanisms in circulating tumor cells of colorectal cancer. Cancer Res 2014;74:1694–1704.
    OpenUrlAbstract/FREE Full Text
  55. 55.↵
    1. Lohr JG,
    2. Adalsteinsson VA,
    3. Cibulskis K,
    4. Choudhury AD,
    5. Rosenberg M,
    6. Cruz-Gordillo P, et al.
    Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer. Nat Biotechnol 2014;32:479–484.
    OpenUrlCrossRefPubMed
  56. 56.↵
    1. Ting DT,
    2. Wittner BS,
    3. Ligorio M,
    4. Vincent Jordan N,
    5. Shah AM,
    6. Miyamoto DT, et al.
    Single-cell RNA sequencing identifies extracellular matrix gene expression by pancreatic circulating tumor cells. Cell Rep 2014;8:1905–1918.
    OpenUrlCrossRefPubMed
  57. 57.↵
    1. Polzer B,
    2. Medoro G,
    3. Pasch S,
    4. Fontana F,
    5. Zorzino L,
    6. Pestka A, et al.
    Molecular profiling of single circulating tumor cells with diagnostic intention. EMBO Mol Med 2014;6:1371–1386.
    OpenUrlAbstract/FREE Full Text
PreviousNext
Back to top

In this issue

Cancer Biology and Medicine: 12 (3)
Cancer Biology & Medicine
Vol. 12, Issue 3
1 Sep 2015
  • Table of Contents
  • Index by author
Print
Download PDF
Email Article

Thank you for your interest in spreading the word on Cancer Biology & Medicine.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Single-cell analyses of circulating tumor cells
(Your Name) has sent you a message from Cancer Biology & Medicine
(Your Name) thought you would like to see the Cancer Biology & Medicine web site.
Citation Tools
Single-cell analyses of circulating tumor cells
Xi-Xi Chen, Fan Bai
Cancer Biology & Medicine Sep 2015, 12 (3) 184-192; DOI: 10.7497/j.issn.2095-3941.2015.0056

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Single-cell analyses of circulating tumor cells
Xi-Xi Chen, Fan Bai
Cancer Biology & Medicine Sep 2015, 12 (3) 184-192; DOI: 10.7497/j.issn.2095-3941.2015.0056
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • CTCs and metastasis
    • Methods for CTC detection and enrichment
    • Current clinical applications of CTCs
    • Single-cell analyses of CTCs
    • Conclusion and perspectives
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • References
  • PDF

Related Articles

  • No related articles found.
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

  • Revisiting strategies to target ABC transporter-mediated drug resistance in CNS cancer
  • Targeting mitochondrial dysfunction to intervene in liver cancer
  • Current progress in neoantigen-based dendritic cell vaccines for solid tumors
Show more Review

Similar Articles

Keywords

  • Circulating tumor cells (CTCs)
  • single cell sequencing
  • metastasis

Navigate

  • Home
  • Current Issue

More Information

  • About CBM
  • About CACA
  • About TMUCIH
  • Editorial Board
  • Subscription

For Authors

  • Instructions for authors
  • Journal Policies
  • Submit a Manuscript

Journal Services

  • Email Alerts
  • Facebook
  • RSS Feeds
  • Twitter

 

© 2025 Cancer Biology & Medicine

Powered by HighWire