The breakthrough in primary human hepatocytes in vitro expansion

PHHs in vitro expansion. Schematic diagram illustrates the expansion of PHHs from diversified individuals to a large quantity, when cultured in HM established by Hui et al,¹⁰ with the addition of Wnt3a and removal of Rspo1, Noggin and forskolin. The ProliHHs maintained a bi-phenotypic status between primary hepatocytes and liver progenitor cells can mature after inducing re-differentiation by 3D culture and repopulate when transplanted into the liver of immuno-deficient Fah knockout mice indicated. Undoubtedly, this will provide a useful model system for disease modeling such as hepatotropic virus infection, as well as for new drug safety assessment and toxicity testing in vitro and paved the way for hepatocyte transplantation therapy to be used on liver disease patients in the future.