Recent advances in single-molecule detection and single-molecule spectroscopy at room
temperature by laser-induced fluorescence offer new tools for the study of individual …

We extend the sensitivity of fluorescence resonance energy transfer (FRET) to the single
molecule level by measuring energy transfer between a single donor fluorophore and a single …

Dynamic structural changes of macromolecules undergoing biochemical reactions can be
studied using novel single molecule spectroscopy tools. Recent advances in applying such …

Single-molecule measurements provide unique information on heterogeneous populations
of molecules: They give access to the complete distribution of observables (rather than only …

Semiconductor nanocrystals were prepared for use as fluorescent probes in biological
staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a …

We describe the synthesis of water-soluble semiconductor nanoparticles and discuss and
characterize their properties. Hydrophobic CdSe/ZnS core/shell nanocrystals with a core size …

Super-resolution optical microscopy is a rapidly evolving area of fluorescence microscopy
with a tremendous potential for impacting many fields of science. Several super-resolution …

We use alternating-laser excitation to achieve fluorescence-aided molecule sorting (FAMS)
and enable simultaneous analysis of biomolecular structure and interactions at the level of …

Synthetic phytochelatin-related peptides are used as an organic coat on the surface of
colloidal CdSe/ZnS semiconductor nanocrystals synthesized from hydrophobic coordinating …

Fluorescence resonance energy transfer and fluorescence polarization anisotropy are used
to investigate single molecules of the enzyme staphylococcal nuclease. Intramolecular …