[HTML][HTML] General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold

C Vincke, R Loris, D Saerens… - Journal of Biological …, 2009 - ASBMB
Nanobodies, single-domain antigen-binding fragments of camelid-specific heavy-chain only
antibodies offer special advantages in therapy over classic antibody fragments because of …

In vitro analysis and in vivo tumor targeting of a humanized, grafted nanobody in mice using pinhole SPECT/micro-CT

I Vaneycken, J Govaert, C Vincke… - Journal of Nuclear …, 2010 - Soc Nuclear Med
Nanobodies are a novel type of immunoglobulinlike, antigen-binding protein with beneficial
pharmacologic and pharmacokinetic properties that are ideally suited to targeting cellular …

Nanobodies and their potential applications

…, N Devoogdt, P De Pauw, C Vincke… - …, 2013 - Future Medicine
Nanobodies are recombinant, antigen-specific, single-domain, variable fragments of camelid
heavy chain-only antibodies. The innate supremacy of nanobodies as a renewable source …

A bispecific nanobody to provide full protection against lethal scorpion envenoming

…, D Saerens, RB Abderrazek, C Vincke… - The FASEB …, 2010 - Wiley Online Library
Envenoming following scorpion sting is a common emergency in many parts of the world.
Our aim was to ameliorate the current 100‐kDa horse plasma antivenom serum (PAS)‐derived …

Preclinical screening of anti‐HER2 nanobodies for molecular imaging of breast cancer

…, N Devoogdt, N Van Gassen, C Vincke… - The FASEB …, 2011 - Wiley Online Library
Accurate determination of tumor human epidermal growth factor receptor 2 (HER2)‐status in
breast cancer patients is possible via noninvasive imaging, provided adequate tracers are …

Synthesis, preclinical validation, dosimetry, and toxicity of 68Ga-NOTA-anti-HER2 Nanobodies for iPET imaging of HER2 receptor expression in cancer

…, J Heemskerk, M Keyaerts, C Vincke… - Journal of Nuclear …, 2013 - Soc Nuclear Med
Nanobodies are the smallest fully functional antigen-binding antibody fragments possessing
ideal properties as probes for molecular imaging. In this study we labeled the anti–human …

[HTML][HTML] Targeted radionuclide therapy with A 177Lu-labeled anti-HER2 nanobody

M D'Huyvetter, C Vincke, C Xavier, A Aerts… - Theranostics, 2014 - ncbi.nlm.nih.gov
RIT has become an attractive strategy in cancer treatment, but still faces important drawbacks
due to poor tumor penetration and undesirable pharmacokinetics of the targeting vehicles. …

[HTML][HTML] Dual beneficial effect of interloop disulfide bond for single domain antibody fragments

J Govaert, M Pellis, N Deschacht, C Vincke… - Journal of Biological …, 2012 - ASBMB
The antigen-binding fragment of functional heavy chain antibodies (HCAbs) in camelids
comprises a single domain, named the variable domain of heavy chain of HCAbs (VHH). The …

Generation of single domain antibody fragments derived from camelids and generation of manifold constructs

C Vincke, C Gutiérrez, U Wernery, N Devoogdt… - … : Methods and Protocols …, 2012 - Springer
Immunizing a camelid (camels and llamas) with soluble, properly folded proteins raises an
affinity-matured immune response in the unique camelid heavy-chain only antibodies (HCAbs…

Ultrasensitive Measurement of Ca2+ Influx into Lipid Vesicles Induced by Protein Aggregates

…, DC Wirthensohn, SF Lee, C Vincke… - Angewandte Chemie …, 2017 - Wiley Online Library
To quantify and characterize the potentially toxic protein aggregates associated with
neurodegenerative diseases, a high‐throughput assay based on measuring the extent of aggregate…