Direct interaction of the mitochondrial membrane protein carnitine palmitoyltransferase I with Bcl-2

M B Paumen; Y Ishida; H Han; M Muramatsu; Y Eguchi; Y Tsujimoto; T Honjo

doi:10.1006/bbrc.1997.6089

Direct interaction of the mitochondrial membrane protein carnitine palmitoyltransferase I with Bcl-2

Biochem Biophys Res Commun. 1997 Feb 24;231(3):523-5. doi: 10.1006/bbrc.1997.6089.

Authors

M B Paumen¹, Y Ishida, H Han, M Muramatsu, Y Eguchi, Y Tsujimoto, T Honjo

Affiliation

¹ Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.

PMID: 9070836
DOI: 10.1006/bbrc.1997.6089

Abstract

To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in coimmunoprecipitations using two different kinds of anti-Bcl-2 antibodies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis*
Carnitine O-Palmitoyltransferase / metabolism*
Intracellular Membranes / metabolism
Membrane Proteins / metabolism*
Mice
Mitochondria / metabolism*
Precipitin Tests
Protein Binding
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Recombinant Proteins
Structure-Activity Relationship

Substances

Membrane Proteins
Proto-Oncogene Proteins c-bcl-2
Recombinant Proteins
Carnitine O-Palmitoyltransferase