Molecular cloning of two novel rab genes from human melanocytes

D Chen; J Guo; T Miki; M Tachibana; W A Gahl

doi:10.1016/0378-1119(96)00509-4

Molecular cloning of two novel rab genes from human melanocytes

Gene. 1996 Sep 26;174(1):129-34. doi: 10.1016/0378-1119(96)00509-4.

Authors

D Chen¹, J Guo, T Miki, M Tachibana, W A Gahl

Affiliation

¹ Section on Human Biochemical Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-1830, USA.

PMID: 8863739
DOI: 10.1016/0378-1119(96)00509-4

Abstract

We isolated the genes of two small GTP-binding proteins of the rab family from a human melanocyte cDNA library and from melanoma cells. One gene, rab30 codes for a novel rab protein of 203 amino acids with minimal homology to previously documented GTPases. The other, rab22b, appears to be an isoform of the human homologue of canine rab22. Both rab mRNAs displayed a nearly ubiquitous pattern of expression in the various tissues examined. Rab22b and rab30 were mapped to chromosomes 18 and 11, respectively.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular*
Dogs
GTP Phosphohydrolases / genetics
GTP-Binding Proteins / genetics*
Humans
Melanocytes / chemistry*
Melanoma / genetics*
Molecular Sequence Data
Sequence Homology
Tumor Cells, Cultured
rab GTP-Binding Proteins*

Substances

RAB22A protein, human
RAB31 protein, human
rab22 protein, Canis familiaris
RAB30 protein, human
GTP Phosphohydrolases
GTP-Binding Proteins
rab GTP-Binding Proteins

Associated data

GENBANK/U57091
GENBANK/U57092