Circular RNA profile identifies circPVT1 as a proliferative factor and prognostic marker in gastric cancer

Jie Chen; Yan Li; Qiupeng Zheng; Chunyang Bao; Jian He; Bin Chen; Dongbin Lyu; Biqiang Zheng; Yu Xu; Ziwen Long; Ye Zhou; Huiyan Zhu; Yanong Wang; Xianghuo He; Yingqiang Shi; Shenglin Huang

doi:10.1016/j.canlet.2016.12.006

Circular RNA profile identifies circPVT1 as a proliferative factor and prognostic marker in gastric cancer

Cancer Lett. 2017 Mar 1:388:208-219. doi: 10.1016/j.canlet.2016.12.006. Epub 2016 Dec 13.

Authors

Jie Chen¹, Yan Li¹, Qiupeng Zheng², Chunyang Bao¹, Jian He¹, Bin Chen¹, Dongbin Lyu¹, Biqiang Zheng¹, Yu Xu¹, Ziwen Long¹, Ye Zhou¹, Huiyan Zhu¹, Yanong Wang¹, Xianghuo He¹, Yingqiang Shi³, Shenglin Huang⁴

Affiliations

¹ Department of Gastric Cancer and Soft Tissue Sarcomas, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
² Department of Gastric Cancer and Soft Tissue Sarcomas, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China; State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200032, China.
³ Department of Gastric Cancer and Soft Tissue Sarcomas, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. Electronic address: yingqiangshi@126.com.
⁴ Department of Gastric Cancer and Soft Tissue Sarcomas, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. Electronic address: slhuang@fudan.edu.cn.

PMID: 27986464
DOI: 10.1016/j.canlet.2016.12.006

Abstract

Circular RNAs (circRNAs) comprise a novel class of widespread non-coding RNAs that may regulate gene expression in eukaryotes. However, the characterization and function of circRNAs in human cancer remain elusive. Here we identified at least 5500 distinct circRNA candidates and a series of circRNAs that are differentially expressed in gastric cancer (GC) tissues compared with matched normal tissues. We further characterized one circRNA derived from the PVT1 gene and termed it as circPVT1. The expression of circPVT1 is often upregulated in GC tissues due to the amplification of its genomic locus. circPVT1 may promote cell proliferation by acting as a sponge for members of the miR-125 family. The level of circPVT1 was observed as an independent prognostic marker for overall survival and disease-free survival of patients with GC. Our findings suggest that circPVT1 is a novel proliferative factor and prognostic marker in GC.

Keywords: Circular RNA; Gastric cancer; Non-coding RNA; Prognosis; circPVT1.

MeSH terms

Adenocarcinoma / genetics*
Adenocarcinoma / pathology
Biomarkers, Tumor / genetics*
Cell Proliferation / genetics
Female
Humans
Male
Middle Aged
Prognosis
RNA / genetics*
RNA, Circular
RNA, Long Noncoding / genetics*
Stomach Neoplasms / genetics*
Stomach Neoplasms / pathology

Substances

Biomarkers, Tumor
PVT1 long-non-coding RNA, human
RNA, Circular
RNA, Long Noncoding
RNA