Abstract
In this study, we detected miR-206 expression in gastric cancer (GC) and further investigated its effects on GC cell growth in vitro and in vivo. miR-206 expression was found to be significantly decreased in 30 GC samples and GC cell lines by real time-PCR. Restoration of miR-206 reduced cell growth and colony forming ability in GC cells with G0/G1 cell cycle arrest. Further studies demonstrated that miR-206 could suppress GC cells proliferation at least partially through targeting the cyclinD2 (CCND2). Therefore, we provided evidence that miR-206 was a potential tumor suppressor and may be used as a therapeutic target for gastric cancer.
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism*
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Adenocarcinoma / pathology
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Animals
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Binding Sites
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Cell Line, Tumor
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Cell Proliferation*
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Cyclin D2 / genetics
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Cyclin D2 / metabolism*
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Down-Regulation
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Female
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G1 Phase Cell Cycle Checkpoints
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Humans
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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MicroRNAs / metabolism*
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Middle Aged
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Oligonucleotides / metabolism
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Promoter Regions, Genetic
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Real-Time Polymerase Chain Reaction
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Resting Phase, Cell Cycle
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Reverse Transcriptase Polymerase Chain Reaction
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Signal Transduction
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Stomach Neoplasms / genetics
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Stomach Neoplasms / metabolism*
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Stomach Neoplasms / pathology
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Transfection
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Tumor Burden
Substances
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CCND2 protein, human
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Cyclin D2
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MIRN206 microRNA, human
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MicroRNAs
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Oligonucleotides