Abstract
Recent studies have revealed that microRNA-29c (miR-29c) is involved in a variety of biological processes including carcinogenesis. Here, we report that miR-29c was significantly downregulated in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) cell lines as well as in clinical tissues compared with their corresponding controls. Tumor necrosis factor alpha-induced protein 3 (TNFAIP3), a key regulator in inflammation and immunity, was found to be inversely correlated with miR-29c levels and was identified as a target of miR-29c. Overexpression of miR-29c in HepG2.2.15 cells effectively suppressed TNFAIP3 expression and HBV DNA replication as well as inhibited cell proliferation and induced apoptosis. We conclude that miR-29c may play an important role as a tumor suppressive microRNA in the development and progression of HBV-related HCC by targeting TNFAIP3. Thus miR-29c and TNFAIP3 represent key diagnostic markers and potential therapeutic targets for the prevention and treatment of HBV infection.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Apoptosis
-
Biomarkers, Tumor / genetics
-
Biomarkers, Tumor / metabolism
-
Carcinoma, Hepatocellular / genetics
-
Carcinoma, Hepatocellular / pathology
-
Carcinoma, Hepatocellular / virology*
-
Cell Line, Tumor
-
Cell Proliferation
-
Cell Transformation, Neoplastic / genetics
-
DNA-Binding Proteins
-
Down-Regulation
-
Gene Expression Regulation, Neoplastic*
-
Hepatitis B Surface Antigens / metabolism
-
Hepatitis B e Antigens / metabolism
-
Hepatitis B virus* / genetics
-
Hepatitis B virus* / metabolism
-
Hepatitis B virus* / physiology
-
Humans
-
Intracellular Signaling Peptides and Proteins / genetics*
-
Liver Neoplasms / genetics
-
Liver Neoplasms / pathology
-
Liver Neoplasms / virology*
-
MicroRNAs / biosynthesis*
-
Nuclear Proteins / genetics*
-
Transfection
-
Tumor Necrosis Factor alpha-Induced Protein 3
Substances
-
Biomarkers, Tumor
-
DNA-Binding Proteins
-
Hepatitis B Surface Antigens
-
Hepatitis B e Antigens
-
Intracellular Signaling Peptides and Proteins
-
MIRN29a microRNA, human
-
MicroRNAs
-
Nuclear Proteins
-
TNFAIP3 protein, human
-
Tumor Necrosis Factor alpha-Induced Protein 3