The aim of this study was to detect alpha-fetoprotein (AFP) mRNA levels in the preoperative and intraoperative peripheral blood of patients with hepatocellular carcinoma (HCC) receiving orthotopic liver transplantation (OLT).
Methods: We detected AFP mRNA by TaqMan real-time reverse transcriptase-polymerase chain reactions (RT-PCR) on the peripheral blood cells (PBCs) from 30 HCC patients undergoing OLT.
Results: In RT-PCR, fluorescence was undetectable in any control. The positive expression rate of AFP mRNA was 23% (7 of 30) in PBC samples of OLT patients preoperatively and 50% (15 of 30) just before hepatectomy during the operation. The positive rate of AFP mRNA in OLT patients at this time was higher than that at preoperation, a difference that was statistically significant (P < .01).
Conclusion: The OLT operation induced release of cells from the liver into the peripheral blood circulation. This may be an important mechanism of liver cancer cell dissemination deserving further investigation.