Abstract
BEAMing allows the one-to-one conversion of a population of DNA fragments into a population of beads. We used rolling circle amplification to increase the number of copies bound to such beads by more than 100-fold. This allowed enumeration of mutant and wild-type sequences even when they were present at ratios less than 1:10,000 and was sensitive enough to directly quantify the error rate of DNA polymerases used for PCR.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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DNA / analysis*
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DNA / genetics
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DNA / metabolism
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DNA Mutational Analysis / methods*
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DNA-Directed DNA Polymerase / metabolism
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Emulsions / chemistry
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Exons / genetics
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Flow Cytometry
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Fluorescent Dyes / chemistry
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Microscopy, Fluorescence
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Microspheres
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Mutation / genetics
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Nucleic Acid Amplification Techniques / methods*
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Oils / chemistry
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Polymerase Chain Reaction / methods
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Reproducibility of Results
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Tumor Suppressor Protein p53 / genetics
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Water / chemistry
Substances
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Emulsions
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Fluorescent Dyes
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Oils
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Tumor Suppressor Protein p53
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Water
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DNA
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DNA-Directed DNA Polymerase