Abstract
HMGB1 is an architectural factor that enhances the DNA binding affinity of several proteins. We have investigated the influence of HMGB1 on DNA binding by members of the Rel family. HMGB1 enhances DNA binding by p65/p50 and p50/p50, but reduces binding by p65/p65, c-Rel/c-Rel, p65/c-Rel, and p50/c-Rel. In pull-down assays, HMGB1 interacts directly with the p50 subunit via its HMG boxes and this interaction is weakened by the presence of the acidic tail. Functionally, HMGB1 is required for the NF-kappaB-dependent expression of the adhesion molecule VCAM-1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Dimerization
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Fibroblasts / metabolism*
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Gene Expression / drug effects
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HMGB1 Protein / metabolism*
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Mice
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NF-kappa B / metabolism*
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Proto-Oncogene Proteins c-rel / metabolism*
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RNA, Messenger / biosynthesis
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RNA, Messenger / drug effects
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Tumor Necrosis Factor-alpha / pharmacology
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Vascular Cell Adhesion Molecule-1 / biosynthesis
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Vascular Cell Adhesion Molecule-1 / genetics
Substances
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HMGB1 Protein
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NF-kappa B
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Proto-Oncogene Proteins c-rel
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Vascular Cell Adhesion Molecule-1