Abstract
In retroviral gene transduction, the efficiency of viral infection was reduced by the proteoglycans and some other materials secreted by the producer lines. In order to remove these inhibitors we have developed the rFN-CH-296-facilitated protocol. Because the rFN-CH-296 molecule has strong ability to bind a retroviral vector, rFN-CH-296 bound plates are utilized to wash out the unbound putative inhibitors present in a virus supernatant. The gene transduction efficiencies of human CD34(+)CD38(-) BMCs with a GALV-pseudotyped vector and the rFN-CH-296-facilitated protocol were compared with the protocol without a coating plate with CH-296, the mean gene transduction efficiencies being found to be 95.5 and 71.1%, respectively.
MeSH terms
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ADP-ribosyl Cyclase
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ADP-ribosyl Cyclase 1
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Animals
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Antigens, CD*
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Antigens, CD34 / metabolism
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Antigens, Differentiation / metabolism
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Binding Sites / physiology
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Bone Marrow Cells / metabolism*
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Bone Marrow Cells / virology
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Fibronectins / metabolism*
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Gene Transfer Techniques
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Genetic Vectors / genetics*
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Genetic Vectors / metabolism*
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Humans
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Leukemia Virus, Gibbon Ape / genetics*
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Membrane Glycoproteins
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Mice
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NAD+ Nucleosidase / metabolism
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Peptide Fragments / metabolism
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Proteoglycans / metabolism
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Recombinant Proteins / metabolism
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Retroviridae Infections / metabolism
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Transduction, Genetic*
Substances
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Antigens, CD
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Antigens, CD34
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Antigens, Differentiation
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Fibronectins
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Membrane Glycoproteins
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Peptide Fragments
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Proteoglycans
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Recombinant Proteins
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ADP-ribosyl Cyclase
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CD38 protein, human
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Cd38 protein, mouse
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NAD+ Nucleosidase
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ADP-ribosyl Cyclase 1