Abstract
Human breast cancer cell line Hs578T was stably transfected with cDNA for cyclooxygenase-1 or -2. When the cells overexpressing cyclooxygenase-1 or -2 were stimulated with concanavalin A, the processing of matrix metalloproteinase-2 was observed with the aid of gelatin zymography. This processing was not seen in mock-transfected and original cells which did not express detectable cyclooxygenase activity. Furthermore, Northern blotting showed 8-13 fold induction of membrane-type 1 matrix metalloproteinase which processed matrix metalloproteinase-2 in the cells expressing cyclooxygenases. These findings suggest that both isoforms of cyclooxygenase mediate the processing of matrix metalloproteinase-2 through induction of membrane-type I metalloproteinase in breast cancer cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Breast Neoplasms
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Concanavalin A / pharmacology
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Cyclooxygenase 1
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Cyclooxygenase 2
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DNA, Complementary
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Enzyme Activation
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Neoplastic / drug effects
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Humans
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Isoenzymes / genetics*
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Matrix Metalloproteinase 1 / genetics
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Matrix Metalloproteinase 2 / genetics
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Matrix Metalloproteinase 2 / metabolism*
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Membrane Proteins
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Mutation
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Prostaglandin-Endoperoxide Synthases / genetics*
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RNA, Messenger / metabolism
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Transfection
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Tumor Cells, Cultured
Substances
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DNA, Complementary
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Isoenzymes
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Membrane Proteins
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RNA, Messenger
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Concanavalin A
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Cyclooxygenase 1
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Cyclooxygenase 2
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PTGS1 protein, human
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PTGS2 protein, human
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Prostaglandin-Endoperoxide Synthases
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 1