Elsevier

Neoplasia

Volume 6, Issue 5, September–October 2004, Pages 611-622
Neoplasia

Gene Expression Profiling of Microdissected Pancreatic Ductal Carcinomas Using High-Density DNA Microarrays1,3

https://doi.org/10.1593/neo.04295Get rights and content
Under a Creative Commons license
open access

Abstract

Pancreatic ductal adenocarcinoma (PDAC) remains an important cause of malignancy-related death and is the eighth most common cancer with the lowest overall 5-year relative survival rate. To identify new molecular markers and candidates for new therapeutic regimens, we investigated the gene expression profile of microdissected cells from 11 normal pancreatic ducts, 14 samples of PDAC, and 4 well-characterized pancreatic cancer cell lines using the Affymetrix U133 GeneChip set. RNA was extracted from microdissected samples and cell lines, amplified, and labeled using a repetitive in vitro transcription protocol. Differentially expressed genes were identified using the significance analysis of microarrays program. We found 616 differentially expressed genes. Within these, 140 were also identified in PDAC by others, such as Galectin-1, Galectin-3, and MT-SP2. We validated the differential expression of several genes (e.g., CENPF, MCM2, MCM7, RAMP, IRAK1, and PTTG1) in PDAC by immunohistochemistry and reverse transcription polymerase chain reaction. We present a whole genome expression study of microdissected tissues from PDAC, from microdissected normal ductal pancreatic cells and pancreatic cancer cell lines using highdensity microarrays. Within the panel of genes, we identified novel differentially expressed genes, which have not been associated with the pathogenesis of PDAC before.

Keywords

Pancreatic cancer
microarray
microdissection
IRAK1
MCM7

Cited by (0)

1

The data set can be obtained through http://vtg.uniklinikum-dresden.de/Pankreasiabor.

3

This paper was supported by Deutsche Krebshilfe (70-2937-Sal).

2

Robert Grützmann, Christian Pilarsky, and Ole Ammerpohl contributed equally.