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  • Oncogenomics
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Profiling, comparison and validation of gene expression in gastric carcinoma and normal stomach

Oncogene volume 22, pages 4287–4300 (2003)Cite this article

Abstract

Gastric carcinoma is the fourth most common cause of cancer death worldwide but its molecular biology is poorly understood. We catalogued the genes expressed in two gastric adenocarcinomas and normal stomach, using serial analysis of gene expression (SAGE), and compared the profiles on-line with other glandular epithelia. Candidates were validated by Northern blotting and immunohistochemistry. A total of 29 480 transcripts, derived from 10 866 genes, were identified. In all, 1% of the genes were differentially expressed (⩾fivefold difference plus P-value ⩽0.01) between cancers and normal stomach. The most abundant transcripts included ribosomal and mitochondrial proteins, of which most were upregulated in the tumours, as were other widely expressed genes including transcription factors, signalling molecules (serine/threonine protein kinases), thymosin beta 10 and collagenase I. Transcripts abundant in normal stomach were functionally important, including gastrin, immunoglobulin alpha, lysozyme, MUC5, pS2 and pepsinogens, which were among 55 gastric-specific genes. Many transcripts were minimally characterized or new, some cancer-associated genes reflected their intestinal morphology, and some normal gastric genes had previously been considered as pancreatic carcinoma markers. The gastric carcinoma profiles resembled other tumours', supporting the existence of common cancer-associated targets. These data provide a catalogue from which to develop markers for better diagnosis and therapy of gastric carcinoma.

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Acknowledgements

We thank Mr Robert McFarlane for sequencing services; Dr Kenneth W Kinzler for SAGE software and advice; Cancer Research UK and the University of Glasgow for research funding; and the Association of Clinical Pathologists, Keystone Symposia and Pfizer Limited for travel grants.

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Authors and Affiliations

  1. Cancer Research UK Department of Medical Oncology, Cancer Research UK Beatson Laboratories, University of Glasgow, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK

    Karin A Oien & W Nicol Keith

  2. University Department of Pathology, Glasgow Royal Infirmary, 84 Castle Street, Glasgow, G4 OSF, UK

    Karin A Oien & Ian Downie

  3. Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK

    J Keith Vass

  4. Department of Surgery, Gartnavel General Hospital, 1053 Great Western Road, Glasgow, G12 0YN, UK

    Grant Fullarton

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  1. Karin A Oien
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  2. J Keith Vass
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  3. Ian Downie
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Correspondence to Karin A Oien.

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Note added in proof

Since our manuscript was submitted, a further relevant and interesting paper has been published. This used SAGE to study one gastric adenocarcinoma, of proximal location (from the gastro-oesophageal junction), plus normal gastric body mucosa. These are different from our SAGE tissue samples, but are also from the stomach. (El-Rifai W, Moskaluk CA, Abdrabbo MK, Harper J, Yoshida C, Riggins GJ, Frierson HF and Powell SM. (2002). Cancer Res., 62, 6823–6826).

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Oien, K., Vass, J., Downie, I. et al. Profiling, comparison and validation of gene expression in gastric carcinoma and normal stomach. Oncogene 22, 4287–4300 (2003). https://doi.org/10.1038/sj.onc.1206615

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