Cancer Letters

Cancer Letters

Volume 184, Issue 2, 28 October 2002, Pages 197-206
Cancer Letters

Identification of genes differentially expressed between gastric cancers and normal gastric mucosa with cDNA microarrays

https://doi.org/10.1016/S0304-3835(02)00197-0Get rights and content

Abstract

To identify genes whose alterations lead to gastric cancer, gene expression profiles have been obtained from 22 gastric cancer tissues and their surrounding gastric mucosa tissues. A total of 16 genes were differentially expressed in more than 50% of gastric cancer tissues compared with surrounding gastric mucosa tissues. Genes such as HMG-Y, fibroblast collagenase inhibitor, and osteopontin are among those that are overexpressed in over 50% of the gastric cancer tissues. Dihydrodiol dehydrogenase, ribonuclease A, and glutathione peroxidase are among those genes that are underexpressed in over 50% of the gastric cancer tissues. We identified genes that are associated with clinical phenotypes of patients with gastric cancers. Alpha-II spectrin, Na/K-ATPase and KIAA0111 are those that are enhanced in intestinal type of gastric cancer. Gene such as platelet-endothelial tetraspan antigen 3 was enhanced in highly metastatic gastric cancer tissues.

Introduction

Gastric cancer is one of the most common cancers worldwide. Gastric cancer, in general, is resistant to chemo- and radiation therapy. Surgery remains the only curative treatment. Analysis of genes whose alteration leads to gastric cancer is essential for understanding and development of therapeutics for gastric cancer. So far, only a few genes have been associated with gastric carcinogenesis. These include c-met, c-erbB2, K-sam, and E-cadherin [1], [2], [3]. Microarray technologies have emerged as key tools for genomic expression analysis for the purpose of studying disease states, identifying drug targets, and profiling time-, tissue- or stage-dependent changes [4], [5]. Identification and characterization of genes that are differentially expressed in gastric cancers compared with their corresponding mucosa tissues is a prerequisite not only for diagnosis but also for development of anticancer drugs. With the advent of current cDNA microarray, we can examine expression profiles of various cancer tissues simultaneously on a genomic scale.

In this study, we exploited cDNA microarray analysis to identify genes that are differentially expressed in gastric cancer tissues compared with surrounding gastric mucosa tissues. We also identified genes that are associated with clinical phenotypes of patients.

Section snippets

Tissues

Gastric cancer tissues were obtained with informed consent from patients who underwent surgical operation at Seoul National University Hospital (Seoul, South Korea). Cancerous and their surrounding gastric mucosa tissues were obtained from same patients, and were immediately frozen in liquid nitrogen. Histological grading of gastric cancer tissues was decided according to the World Health Organization (WHO) classification. Tumor content of each gastric cancer tissue ranges between 60 and 70%

Identification of genes that are differentially expressed in gastric cancer tissues compared with surrounding gastric mucosa tissues

We wanted to identify genes that are associated with gastric cancer. For this, we performed cDNA microarray analysis of 2400 genes to determine genes that are differentially expressed in gastric cancer tissues compared with surrounding gastric mucosa tissues. Table 1 describes the list of gastric cancer tissues. Gastric cancer tissues were obtained from 22 patients who underwent surgical resection. Significant numbers of these tissues are diffuse and poorly differentiated types. We carried out

Discussion

The incidence of gastric cancer has recently declined. However, it is still one of the most common causes of cancer-related deaths in Asian countries. The molecular mechanism of gastric carcinogenesis is not well understood because of lack of sufficient information on genetic alterations leading to gastric carcinogenesis. Through cDNA microarray analysis, we identified genes that are differentially expressed in gastric cancer tissues compared with their surrounding gastric mucosa tissues.

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  • Cited by (0)

    1

    The first two authors contributed equally to this paper.

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