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Staphylococcal entorotoxin B anchored exosome induces apoptosis in negative esterogen receptor breast cancer cells

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Tumor Biology

Abstract

Exosomes (EXO) are acellular vehicles used for cancer immunotherapy due to their immune inducing properties. To identify whether designed structure based on tumoral EXO have a cytotoxic effect together with a potent immunological property, we synthesized a novel structure based on EXO and staphylococcal entrotoxin B (SEB), two immune inducer substances, and surveyed its cytostatic effect on the breast cancer cell line. EXO were purified from tumor cells and SEB was anchored on it by protein transfer method. To determine the cytotoxic and apoptosis inducing effect of this structure, treated cells with different concentrations of EXO/SEB were examined by MTT assay and Hoechst staining method. In addition, the expression rate of bcl-2, bax, bak, fas, bcl-xl and the activity of caspase-3 and caspase-9 were assessed. We observed that EXO/SEB significantly decreased the cell proliferation and stimulated apoptosis (P < 0.001) at all concentration after 24 h (P < 0.001). Furthermore, EXO/SEB raised the expression rate of bax and bak (P < 0.001) but no impact on fas and bcl-xl after 48 h. We observed reducing effect of EXO/SEB on the mRNA expression of bcl-2. After 24 h of exposing the cell with the EXO/SEB, a significant increase was found in the activity of caspase at the concentration of 2.5, 5 and 10 μg/100 μl for caspase-9 and at all concentrations for caspase-3 (P < 0.001). Our designed structure, the EXO/SEB, is a novel model for apopto-immunotherapy being able to induce apoptosis in ER− breast cancer cells.

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Acknowledgements

This study was a part of the dissertation of Hamideh Mahmoodzadeh Hosseinini, submitted to Tabriz University of Medical Sciences in partial fulfillment of the requirements for PhD in pharmaceutical biotechnology (No.78). This work was supported by the grant from the Iranian National Sciences Foundation (INSF) and Tabriz University of Medical Sciences.

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The authors confirm that this article content has no conflict of interest.

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Authors and Affiliations

  1. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Hamideh Mahmoodzadeh Hosseini

  2. Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Abbas Ali Imani Fooladi

  3. Anatomical Sciences Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Jafar Soleimanirad

  4. Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Mohammad Reza Nourani

  5. Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran

    Soodabeh Davaran

  6. Department of virology, Pasteur Institute of Iran, Tehran, Iran

    Mehdi Mahdavi

Authors
  1. Hamideh Mahmoodzadeh Hosseini
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  2. Abbas Ali Imani Fooladi
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  3. Jafar Soleimanirad
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  4. Mohammad Reza Nourani
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  6. Mehdi Mahdavi
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Correspondence to Abbas Ali Imani Fooladi.

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Mahmoodzadeh Hosseini, H., Imani Fooladi, A.A., Soleimanirad, J. et al. Staphylococcal entorotoxin B anchored exosome induces apoptosis in negative esterogen receptor breast cancer cells. Tumor Biol. 35, 3699–3707 (2014). https://doi.org/10.1007/s13277-013-1489-1

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  • DOI: https://doi.org/10.1007/s13277-013-1489-1

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