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RETRACTED ARTICLE: miR-124 Acts as a Tumor Suppressor in Glioblastoma via the Inhibition of Signal Transducer and Activator of Transcription 3

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This article was retracted on 13 July 2017

Abstract

MicroRNAs are important regulators of multiple cellular processes, and aberrant miRNA expression has been observed in human glioblastoma (GBM). The present study was to evaluate the level of miR-124 and signal transducer and activator of transcription 3 (STAT3) in GBM tissues and cells. We further investigated the molecular mechanisms of miR-124 and STAT3 in GBM cell lines U87 and U251. Here, we found that miR-124 expression was downregulated in GBM tissues and U87 and U251 cells (all p < 0.001) but not associated with blood routine (RBC, WBC count, etc.) and liver and renal function indicators (all p > 0.05). By contrast, STAT3 was upregulated. Furthermore, the expression of miR-124 was inversely proportional to that of STAT3 mRNA or protein (p = 0.013, p = 0.015, respectively). In vitro studies demonstrated that the overexpression of miR-124 played a suppressor role in the proliferation of U87 and U251 cells and promoted cell apoptosis. Luciferase reporter assays confirmed that miR-124 binding to the 3′-UTR regions of STAT3 inhibited the expression of STAT3 in U87 and U251 cells. However, the inhibitor of miR-124 promoted the expression of STAT3 and cell proliferation. In conclusion, our data suggest that miR-124 may have a potential role in treatment of GBM patients and that miR-124 is a novel regulator of invasiveness and tumorigenicity in GBM cells by targeting STAT3. The miR-124/STAT3 pathway may be a useful therapeutic agent in GBM patients.

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Acknowledgements

We thank all the patients who were willing to be recruited in this investigation.

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Authors and Affiliations

  1. Department of Laboratory Medicine, Second Hospital of Hebei Medical University, No. 215 Hepingxi Road, Shijiazhuang, Hebei, 050000, China

    Weihao Li, Junhua Su, Xin Ji, Xiaobin Zhang & Zhiping Zhang

  2. Department of Laboratory Medicine, Children’s Hospital of Hebei Province, Shijiazhuang, Hebei, China

    Hui Huang

  3. Department of Blood Transfusion, Hebei Provincial Chest Hospital, Shijiazhuang, Hebei, China

    Hong Wang

Authors
  1. Weihao Li
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  2. Hui Huang
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  3. Junhua Su
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  4. Xin Ji
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  5. Xiaobin Zhang
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  6. Zhiping Zhang
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  7. Hong Wang
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Corresponding author

Correspondence to Weihao Li.

Ethics declarations

Human tissue samples used in this study were obtained with written informed consent from the patients or their relatives. This study was approved by the Ethics Committee of Second Hospital of Hebei Medical University.

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None.

Additional information

This article has been retracted at the request of the Editor-in-Chief and the Publisher per the Committee on Publication Ethics guidelines. The article shows evidence of irregularities in authorship during the submission process, there is strong reason to believe that the peer review process was compromised and the authors have plagiarized parts from the following article:

Zhen Li, Hua Wang, Zhiqiang Wang, Hongbing Cai, MiR-195 inhibits the proliferation of human cervical cancer cells by directly targeting cyclin D1, Tumor Biol. (2016) 37:6457-6463, DOI 10.1007/s13277-015-4540-6, Received: 4 November 2015

As such the validity of the content of this article cannot be verified.

An erratum to this article is available at http://dx.doi.org/10.1007/s12035-017-0682-4.

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Li, W., Huang, H., Su, J. et al. RETRACTED ARTICLE: miR-124 Acts as a Tumor Suppressor in Glioblastoma via the Inhibition of Signal Transducer and Activator of Transcription 3. Mol Neurobiol 54, 2555–2561 (2017). https://doi.org/10.1007/s12035-016-9852-z

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  • DOI: https://doi.org/10.1007/s12035-016-9852-z

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