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Drosophila Bys is nuclear and shows dynamic tissue-specific expression during development

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Abstract

Although the bys-like family of genes has been conserved from yeast to humans, it is not apparent to what extent the function of Bys-like proteins has been conserved across phylogenetic groups. Human Bystin is thought to function in a novel cell adhesion complex involved in embryo implantation. The product of the yeast bys-like gene, Enp1, is nuclear and has a role in pre-ribosomal RNA (pre-rRNA) splicing and ribosome biogenesis. To gain insight into the function of the Drosophila melanogaster bys-like family member, termed bys, we examined bys mRNA expression and the localization of Bys protein. In embryos, bys mRNA is expressed in a tissue-specific pattern during gastrulation. In the larval wing imaginal disc, bys mRNA is expressed in the ventral and dorsal regions of the wing pouch, regions that give rise to epithelia that adhere to one another after the wing disc everts. The bys mRNA expression patterns could be interpreted as being consistent with a role for Bys in events requiring cell-cell interactions. However, embryonic bys mRNA expression patterns mirror those of genes that are potential targets of the growth regulator Myc and encode nucleolar proteins implicated in cell growth. Additionally, in Schneider line 2 (S2) cells, an epitope-tagged Bys protein is localized to the nucleus, suggesting that Drosophila Bys function may be conserved with that of yeast Enp1.

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Acknowledgements

We thank Hélène Barcelo for suggestions and construction of pPAAT, Delcy Rodriguez and Jerzy Bilski for assistance, Jayma Moore and Tom Freeman for assistance with confocal microscopy, and H. Barcelo, Glen McBride, Carrie Leopold and Ron Hutchison for comments on the manuscript. This work was supported by NIH grants GM60956 and GM067756 to M.J.S. E.K.N. was an AURA fellow funded by ND EPSCoR through NSF grant OSR-9452892.

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Correspondence to Mary J. Stewart.

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Edited by D.A. Weisblat

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Stewart, M.J., Nordquist, E.K. Drosophila Bys is nuclear and shows dynamic tissue-specific expression during development. Dev Genes Evol 215, 97–102 (2005). https://doi.org/10.1007/s00427-004-0447-8

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