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Study of metabonomic profiles of human esophageal carcinoma by use of high-resolution magic-angle spinning 1H NMR spectroscopy and multivariate data analysis

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Abstract

Esophageal carcinoma (EC) is one of the most common malignant tumors. EC survival has remained disappointingly low because of the high malignancy of esophageal cancer and the lack of obvious clinical symptoms at an early stage. Early diagnosis is often difficult because the small tumor nodules are frequently missed. Metabonomics based on high-resolution magic-angle spinning (HRMAS) NMR has been popular for tumor detection because it is highly sensitive, provides rich biochemical information and requires no sample pretreatment. 1H HRMAS spectra of non-involved adjacent esophageal tissues and of well differentiated and moderately differentiated esophageal carcinoma tumors were recorded and analyzed by use of multivariate and statistical analysis techniques. Moderately differentiated EC tumors were found to have increased total choline, alanine, and glutamate and reduced creatine, myo-inositol, and taurine compared with non-involved adjacent tissues. Moreover, clear differences between the metabonomic profiles of EC tissues enabled tumor differentiation. Furthermore, the integral Gly/MI ratio for samples of different tissue types were statistically significantly different; this was sufficient both for distinguishing non-involved tissues from esophageal carcinoma and for classification of well differentiated and moderately differentiated EC tumors.

Tissue metabonomics analysis based on the HRMAS 1H NMR spectroscopy is a powerful nondestructive approach in characterizing the metabolite composition in human esophageal carcinoma (EC), in the development of new diagnostic methods, and perhaps in the evaluation processes of clinical therapies. The result demonstrated that (a) the metabonomes of both well-differentiated EC and moderately differentiated EC tumors differ markedly from that of the adjacent non-involved tissues, and (b) well-differentiated EC tumors have clear differences in metabonome from that of the moderately differentiated EC tumors by using multivariate data analysis

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Acknowledgements

We acknowledge financial support from the National Natural Science Foundation of China (21005022, 81073024, 30900764), the Guangdong Natural Science Foundation (S2011010002512), and the Foundation of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics (T151003). We are also grateful for excellent suggestions and comments made by two anonymous referees which enabled us to further improve the manuscript.

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Authors and Affiliations

  1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Yongxia Yang & Lijing Wang

  2. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Shumei Wang & Shengwang Liang

  3. Zhongshan Campus, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Ali Chen

  4. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan, 430071, China

    Yongxia Yang, Huiru Tang, Lei Chen & Feng Deng

  5. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Yongxia Yang & Lijing Wang

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  1. Yongxia Yang
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Correspondence to Yongxia Yang.

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Yang, Y., Wang, L., Wang, S. et al. Study of metabonomic profiles of human esophageal carcinoma by use of high-resolution magic-angle spinning 1H NMR spectroscopy and multivariate data analysis. Anal Bioanal Chem 405, 3381–3389 (2013). https://doi.org/10.1007/s00216-013-6774-8

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  • DOI: https://doi.org/10.1007/s00216-013-6774-8

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