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Emerging Technologies for CTC Detection Based on Depletion of Normal Cells

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Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer

Part of the book series: Recent Results in Cancer Research ((RECENTCANCER,volume 195))

Abstract

Properly conducted, an enrichment step can improve selectivity, sensitivity, yield, and most importantly, significantly reduce the time needed to isolate rare circulating tumor cells (CTCs). The enrichment process can be broadly categorized as positive selection versus negative depletion, or in some cases, a combination of both. We have developed a negative depletion CTC enrichment strategy that relies on the removal of normal cells using immunomagnetic separation in the blood of cancer patients. This method is based on the combination of magnetic and fluid forces in an axial, laminar flow in long cylinders placed in quadrupole magnets. Using this technology, we have successfully isolated CTCs from patients with breast carcinoma and squamous cell carcinoma of the head and neck. In contrast to a positive selection methodology, this approach provides an unbiased characterization of these cells, including markers associated with epithelial mesenchymal transition.

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Author information

Authors and Affiliations

  1. Internal Medicine, Division of Medical Oncology, The Ohio State University, Columbus, OH, USA

    Maryam Lustberg

  2. Department of Otolaryngology—Head and Neck Surgery, The Ohio State University and Nationwide Children’s Hospital, Columbus, OH, USA

    Kris R. Jatana

  3. Department of Biomedical Engineering, Cleveland Clinic, Cleveland, OH, USA

    Maciej Zborowski

  4. Professor William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Avenue, Columbus, OH, 43210, USA

    Jeffrey J. Chalmers

Authors
  1. Maryam Lustberg
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  2. Kris R. Jatana
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  3. Maciej Zborowski
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  4. Jeffrey J. Chalmers
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Corresponding author

Correspondence to Jeffrey J. Chalmers .

Editor information

Editors and Affiliations

  1. , Breast Cancer Translational Res. Lab., Institut Jules Bordet, Boulevard de Waterloo 125, Brussels, 1000, Belgium

    Michail Ignatiadis

  2. , Breast Cancer Translational Res. Lab., Institut Jules Bordet, Boulevard de Waterloo 125, Brussels, 1000, Belgium

    Christos Sotiriou

  3. Zentrum für Experimentelle Medizin, Inst. Tumorbiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistr. 52, Hamburg, 20246, Germany

    Klaus Pantel

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Lustberg, M., Jatana, K.R., Zborowski, M., Chalmers, J.J. (2012). Emerging Technologies for CTC Detection Based on Depletion of Normal Cells. In: Ignatiadis, M., Sotiriou, C., Pantel, K. (eds) Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer. Recent Results in Cancer Research, vol 195. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28160-0_9

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  • DOI: https://doi.org/10.1007/978-3-642-28160-0_9

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  • Online ISBN: 978-3-642-28160-0

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