Examples of methods for monitoring pyroptosis
| Pyroptosis mechanism | Detection | Reference |
|---|---|---|
| Caspase-3/GSDME | Cell swelling with large bubbles | 23 |
| Cleavage of GSDME | ||
| GSDME-mediated pore formation (liposome leakage method) | ||
| ATP cell viability | ||
| LDH release | ||
| PI/FITC staining | ||
| GZMB/(Caspase-3)/GSDME | The changes in cell morphology | 53 |
| Cleavage of GSDME | ||
| LDH, HMGB1 release | ||
| SYTOX green uptake | ||
| The use of zDEVD-fmk or zVAD-fmk to inhibit apoptosis and caspase-3-mediated pyroptosis | ||
| GZMA/GSDMB | The changes in cell morphology | 51 |
| Cleavage of GSDMB | ||
| Edman sequencing of the N termini to identify the cleavage sites | ||
| ATP–based cell viability LDH release | ||
| PI/FITC staining | ||
| Hypoxia/TNF-α/GSDMC | Cell swelling with large bubbles | 50 |
| Cleavage of GSDMC | ||
| Liposome leakage method | ||
| Cell death determined by LDH release | ||
| SYTOX green staining |