Table 1

Affinity prediction and detection results of wild-type neoepitopes and altered neoepitopes

Position of peptide	Sequences	Prediction algorithms-IEDB	FI^a	DC₅₀^b
mutABCA2	FIGITATVV	3.7	3.32 ± 0.36	<18 h
mutABCA2_L2	FLGITATVV	1.0	4.49 ± 0.42	>18 h
mutSP140	PLLPVTCGV	2.1	3.79 ± 0.50	>24 h
mutSP140_Y1	YLLPVTCGV	0.2	5.20 ± 0.57	>24 h
mutSTOM	SVIISVDGV	4.6	4.27 ± 0.52	<18 h
mutSTOM_L2	SLIISVDGV	1.0	4.71 ± 0.41	>18 h
HIV-1 peptide	ILKEPVHGV	1.8	4.76 ± 0.41	>24 h

^aFI = (average PE fluorescence with the given peptide – average PE fluorescence without peptide)/(average PE fluorescence without peptide). ^bDC₅₀ is defined as the time required for 50% dissociation of the pMHC complex stabilized at t = 0 h.