Summary of multiplex imaging methods, related analytical software, research fields, and new targets discovered via these technologies
| Methods | Analytical software | Research fields | New therapeutic targets |
|---|---|---|---|
| †IMC (Hyperion)1 | CellProfiler, histoCAT, Voronoi diagrams | HCC | Resident macrophages12 |
| Breast carcinoma | Vimentin+Slug− macrophages13 | ||
| Melanoma | B2M14 | ||
| ‡CODEX2 | Volumetric segmentation algorithm by Yury Goltsev et al.2, X-shift | Colorectal cancer | PD-1+CD4+ T cells15 |
| §Immuno-SABER3 | – | Imaging of normal human tissue | – |
| ¶TSA systems (Vectra Polaris)7 | Nuance and InForm image analysis software (PerkinElmer) | HCC | Treg and exhausted CD8 T cells16, PD-18 |
†IMC, imaging mass cytometry; ‡CODEX, co-detection by indexing; §Immuno-SABER, immunostaining with signal amplification by exchange reaction; ¶TSA systems, tyramide signal amplification system.