Reported biomaterial-based platforms for CSC enrichment and study
| Material | Morphology | Cell line for enrichment | W/O cytokines | Reference | |
|---|---|---|---|---|---|
| Natural material | Matrigel | Cell-laden disc | Adenocarcinomic human alveolar basal epithelial cells, A549 Human breast cancer cells, MCF7 Human prostate cancer cells, PC3 | N | 27 |
| HA + chitosan | Disc | Human glioblastoma multiforme, U118 | N | 29 | |
| HA + chitosan | Disc | Human glioma, U87 | N | 30 | |
| Collagen I + PEG | Cell-laden disc | Human hepatocyte cell line, HepG2 | N | 32 | |
| Collagen I + keratin | Disc | Colorectal cancer cell line, HT29 | Y | 33 | |
| Collagen I | Plate | Human breast cancer cells, MCF7 | N | 34 | |
| Alginate | Microcapsule | Human cancer cell line, PANC-1 | N | 36 | |
| Alginate | Microcapsule | Human pancreatic cell line, PC-3 | Y | 17 | |
| Alginate | 3D printed cell-laden plate | Human glioblastoma multiforme, U118 | N | 35 | |
| Alginate | Cell-laden microbeads | Human hepatocellular carcinoma cells, HCCLM3 Human head and neck squamous cell carcinoma cells, TCA8113 | N | 37,38 | |
| Alginate + HA | Disc | Mouse breast cancer cells, 4T1 | Y | 31 | |
| Alginate + chitosan | Disc | Mouse prostate cancer cells, TRAMP-C2 Human breast cancer cells, MDA-MB-231 Human hepatocellular carcinoma, SK-Hep1 | N | 39 | |
| Alginate + chitosan | Disc | Human glioblastoma cells, U87 Human glioblastoma multiforme, U118 | N | 40 | |
| Fibrin gel | Cell-laden disc | Murine melanoma cells, B16-F1 | N | 50 | |
| Synthetic material | PCL | 3D printed disc | Human breast cancer cells, MCF7 | N | 43 |
| PDMS | Disc | Human melanoma cells, WM115 | N | 44 | |
| PEGDA | Cell-laden plate | Mouse breast cancer cells 4T1 Human breast cancer cells, MCF7 | Y | 45 | |
| PAH/HA | Plate | Human hepatocellular carcinoma cells, HUH7 | N | 46 | |
| pV4D4 | Thin film | Human ovarian cancer cell line, SKOV3 Human breast cancer cell line, MCF-7 Human liver carcinoma cell line, Hep3B Human colon cancer cell line, SW480 | N | 47 | |