Table1

Summary of valuable references and guidelines relevant to clinical NGS oncology testing

Source	Title	Content summary	Reference
New York State Board of Health	"Next Generation" Sequencing (NGS) guidelines for somatic genetic variant detection	Detailed standards for technical validity	¹¹
ACMG	Standards and guidelines for the interpretation of sequence Variants	Guidelines for clinical validity assessment, particularly for germline/constitutional variants	¹²
ACMG	ACMG clinical laboratory standards for next-generation sequencing	Broad summaries of major areas of consideration for clinical validation of all NGS assay	¹³
CDC	Assuring the quality of next-generation sequencing in clinical laboratory practice	Detailed recommendations for technical validity assessment/validation of all NGS assays	¹⁴
CDC	Good laboratory practice for clinical next-generation sequencing informatics pipelines	Detailed recommendations for clinical validity assessment for all NGS assays	¹⁵
Quest Diagnostics (reference laboratory)	Annotation of sequence variants in cancer samples processes and pitfalls for routine assays in the clinical laboratory	The framework for a repeatable workflow for clinical validity assessment in use at a high volume testing facility is described	¹⁶

ACMG: American College of Medical Genetics and Genomics; CDC: United States Centers for Disease Control and Prevention