Forward and reverse primers of HLA-DRB1 for PCR and for sequencing.
| Types | Primer for PCR | Primer for Sequencing |
|---|---|---|
| DRB1*01 | 5’-CCC GAC CAC GTT TCT TGT GGC AGC TT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT TGA GC-3’ | F 5’-GAG CGG GTG CGG TTG CTG GAA AGA T-3’ R 5’-GAC ACA ACT ACG GGG TTG GTG AGA G-3’ |
| DRB1*03 | 5’-CCA GAC CAC GTT TCT TGG AGT ACT CT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT CCA TC-3’ | F 5’-GAG CGG GTG CGG TAC CTG GAC AGA T-3’ R 5’-GAC ACA ACT ACG GGG TTG TGG AGA G-3’ |
| DRB1*04 | 5’-CCC GAC CAC GTT TCT TGG AGC AGG TT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT CTA TC-3’ | F 5’-GAG CGG GTG CGG TTC CTG GAC AGA T-3’ R 5’-GAC ACA ACT ACG GGG TTG GTG AGA G-3’ |
| DRB1*07 | 5’-CCC AAC CAC GTT TCC TGT GGC AGG GT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT CCA TC-3’ | F 5’-GAG CGG GTG CAG TTC CTG GAA AGA C-3’ R 5’-GAC ACA ACT ACG GGG TTG GTG AGA G-3’ |
| DRB1*08 | 5’-CCC GAC CAC GTT TCT TGG AGT ACT CT-3’ 5’-CTT CAC GGT GCA GCG GCG AGT CCA TC-3’ | F 5’-GAG CGG GTG CGG TTC CTG GAC AGA T-3’ R 5’-GAC ACA ACT ACG GGG TTG GTG AGA G-3’ |
| DRB1*09 | 5’-CCC AAC CAC GTT TCT TGA AGC AGG AT-3’ 5’-CTT CAC AGT GCA GAG GCG AGT CCA TC-3’ | F 5’-GAG CGG GTG CGG TAT CTG CAC AGA G-3’ R 5’-GAC ACA ACT ACG GGG TTG GTG AGA G-3’ |
| DRB1*10 | 5’-CCA GAC CAC GTT TCT TGG AGG AGG TT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT TCA AC-3’ | F 5’-GAG CGG GTG CGG TTG CTG GAA AGA C-3’ R 5’-GAC ACA ACT ACG GGG TTG GTG AGA G-3’ |
| DRB1*11 | 5’-CCA GAC CAC GTT TCT TGG AGT ACT CT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT CCA TC-3’ | F 5’-GAG CGG GTG CGG TTC CTG GAC AGA T-3’ R 5’-GAC ACA ACT ACG GGG TTG GTG AGA G-3’ |
| DRB1*12 | 5’-CCA GAC CAC GTT TCT TGG AGT ACT CT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT CCA TC-3’ | F 5’-GAG CGG GTG CGG TTA CTG GAG AGA C-3’ R 5’-GAC ACA ACT ACG GGG CTG TGG AGA G-3’ |
| DRB1*13 | 5’-CCA GAC CAC GTT TCT TGG AGT ACT CT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT CCA TC-3’ | F 5’-GAG CGG GTG CGG TTC CTG GAC AGA T-3’ R 5’-GAC ACA ACT ACG GGG TTG TGG AGA G-3’ |
| DRB1*14 | 5’-CCA GAC CAC GTT TCT TGG AGT ACT CT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT CCA TC-3’ | F 5’-GAG CGG GTG CGG TTC CTG GAC AGA T-3’ R 5’-GAC ACA ACT ACG GGG TTG TGG AGA G-3’ |
| DRB1*15 | 5’-CCC GAC CAC GTT TCC TGT GGC AGC CT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT CCA AC-3’ | F 5’-GAG CGG GTG CGG TTC CTG GAC AGA T-3’ R 5’-GAC ACA ACT ACG GGG TTG TGG AGA G-3’ |
| DRB1*16 | 5’-CCC GAC CAC GTT TCC TGT GGC AGC CT-3’ 5’-CTT CAC AGT GCA GCG GCG AGT CCA AC-3’ | F 5’-GAG CGG GTG CGG TTC CTG GAC AGA T-3’ R 5’-GAC ACA ACT ACG GGG TTG GTG AGA G=3’ |