The detection of mRNA of CXCR4 by Real-time PCR.
| The type of cells | Ct(CXCR4) | Ct(GAPDH) | ΔCt | ΔΔCt | 2-ΔΔCt | Inhibition rate (%) |
|---|---|---|---|---|---|---|
| Control-siRNA | 27.29 | 20.16 | 7.13 | 0 | 1 | 0 |
| CXCR4-siRNA1 | 34.32 | 22.67 | 11.65 | 4.52 | 0.043285 | 95.7 |
| CXCR4-siRNA2 | 32.12 | 22.16 | 9.96 | 2.83 | 0.140632 | 85.9 |
| CXCR4-siRNA3 | 35.12 | 22.15 | 12.97 | 5.84 | 0.017458 | 98.3 |