Expression changes of development and differentiation associated genes in the differentiation process from NSCs to astrocytes with a semi-quantitative RT-PCR assay
| Glioma differentiation-associated genes | Undiffercntiated NSC | NSCs Initiating differentiation | Glial progenitor cells | Astrocytes | Control |
|---|---|---|---|---|---|
| ACXCR4 | 0.203 ± 0.011 | 0.325 ± 0.025c | 0.133 ± 0.010c | 0.029 ± 0.032ac | 0.171 ± 0.009 |
| T enascin-(CTN-C) | 0.062 ± 0.005 | 0.199 + 0.006ac | 0.107 ± 0.004a | 0.145 + 0.004ac | 0.045 ± 0.004 |
| GLT1 | 0.099 ± 0.012a | 1.077 ± 0.061c | 0.274 ± 0.020a | 0.091 ± 0.015a | 0.600± 0.062 |
| IL1-RI | 0.022 + 0.007a | 0.115 + 0.008ac | 0.025 ± 0.006a | 0.015 ± 0.005a | 0.246 ± 0.025 |
| EGFR-8 | 0.012 ± 0.006a | 0.350 ± 0.024bc | 0.260 ± 0.027a | 0.305 ± 0.013ac | 0.428 ± 0.020 |
| CDC2 | 0.041 + 0.010a | 0.918 ± 0.019c | 0.699 ± 0.019a | 0.651 ± 0.046ac | 0.946 ± 0.048 |
| Ndr3 | 0.311 ± 0.010b | 0.283 ± 0.018 | 0.173 ± 0.016ac | 0.081 + 0.012ac | 0.231 ± 0.030 |
| MAPKK4 | 0.021 ± 0.008a | 0.185 ± 0.026ac | 0.105 ± 0.013ac | 0.057 ± 0.014ad | 0.274 ± 0.022 |