Table 1.

Primer Sequence and reaction parameters for different genes

Gene Name	Primer Sequence	Product Length	Reaction Parameters
Madl	sense: 5’-TGTGAGCGACTCTGACGA-3’ antisense: 5’-GTGGGACACTGAAGTTTACG -3’	210bp	94”C,30 s; 54”C,30s; 72’C, 1 min
β-actin	sense: 5’-TTGTAACCTTCTGGGACGATATGG-3’ antisense: 5’-TCATAGGCCCCGGTCACATC -3’	650bp	94-C.30 s; 55”C,30s: 72’C, 1 mm
TERT	sense: 5’-AGGTCATTCTTTTACATCAC-3’ antisense: 5’-TCATAGGCCCCGGTCACATC -3’	482bp	94°C,30s; 53V, 30 s; 72V, 1 min

Gene Name

Primer Sequence

Product Length

Reaction Parameters

Madl

sense: 5’-TGTGAGCGACTCTGACGA-3’

antisense: 5’-GTGGGACACTGAAGTTTACG -3’

210bp

94”C,30 s; 54”C,30s; 72’C, 1 min

β-actin

sense: 5’-TTGTAACCTTCTGGGACGATATGG-3’

antisense: 5’-TCATAGGCCCCGGTCACATC -3’

650bp

94-C.30 s; 55”C,30s: 72’C, 1 mm

TERT

sense: 5’-AGGTCATTCTTTTACATCAC-3’

antisense: 5’-TCATAGGCCCCGGTCACATC -3’

482bp

94°C,30s; 53V, 30 s; 72V, 1 min