Well | Control | PP-0.39 | ||
---|---|---|---|---|
12 μg/ml | 24 μg/ml | 36 μg/ml | ||
Average Ct | 26.40 ± 0.084 | 27.17 ± 0.107 | 30.39 ± 0.154 | 31.11 ± 0.09 |
TSR8, amoles | 54.80 ± 6.03 | 1.39 ± 0.201 | 0.279 ± 0.06 | 0.107 ± 0.013 |
% of Control activity | 100 | 2.54 | 0.51 | 0.19 |
HL-60 cells were subcultured at an initial concentration of 105 cells/ml with or without the indicated amount of PP-0.39. After incubation for 3 days, 106 cells from each flask were sedimented for enzyme extraction as described in materials and methods. Aliquots of 2 μl were withdrawn for the assay of telomerase activity as described in Table 3.