Reaction system of real-time PCR.
| Reagent | Quantity (μl) | Concentration |
|---|---|---|
| ddH2O | 5.285 | |
| 10 X buffer | 1.5 | 1 × |
| dNTPs mixture (2.5 mM) | 1.125 | 200 μM |
| Mg2+ | 0.9 | 2.5 mM |
| Eva Green | 0.75 | |
| Fluorescence | 0.09 | |
| Taq DNA polymerase (2.5 μ/μl) | 0.15 | 0.025 μ/μl |
| Primer R (3 μM) | 0.6 | 0.12 μM |
| Primer F (3 μM) | 0.6 | 0.12 μM |
| Template (1:40) | 4.0 | |
| Total volume | 15.0 |