Table 1.

Three genes MSP Primer sequences, annealing temperatures, and PCR product sizes.

Gene		Sequence (5’~3’)	Size (bp)	Temperature (°C)
MGMT	Mf	TTT CGA CGT TCG TAG GTT TTC GC	81	55
	Mr	GCA CTC TTC CGA AAA CGA AAC G	81	55
	Uf	TTT GTG TTT TGA TGT TTG TAG GTT TTT GT	93	63
	Ur	AAC TCC ACA CTC TTC CAA AAA CAA AAC A	93	63
hMLHl	Mf	ACG TAG ACG TTT TAT TAG GGT CGC	115	60
	Mr	CCT CAT CGT AAC TAC CCG CG
	Uf	TTT TGA TGT AGA TGT TTT ATT AGG GTT GT	124	63
	Ur	ACC ACC TCA TCA TAA CAT CCC ACA
hMSH2	Mf	TCG TGG TCG GAC GTC GTT C	134	60
	Mr	CAA CGT CTC CTT CGA CTA CAC CG	134	60
	Uf	GGT TGT TGT GGT TGG ATG TTG TTT	143	63
	Ur	CAA CTA CAA CAT CTC CTT CAA CTA CAC CA	143	63