Primer pairs used for PCR-amplified mtRNA
| Gene | Primer | Sequence | Length (mer) | Tm (°C) | GC% | Product size (bp) |
|---|---|---|---|---|---|---|
| COI | F6043 | TCTAGGTAACGACCACATCTACAAC | 25 | 62.4 | 44.0 | 614 |
| R6656 | CGAAGCCTGGTAGGATAA | 18 | 51.9 | 50.0 | ||
| ATPase6 | F9000 | CGCCTAACCGCTAACATTACTG | 22 | 64.2 | 50.0 | 148 |
| R9147 | AGGCGACAGCGATTTCTA | 18 | 53.8 | 50.0 | ||
| ND4 | F11581 | ATCTGCCTACGACAAACA | 18 | 48.3 | 44.4 | 443 |
| R12024 | GTGGTGGGTGAGTGAGCCC | 19 | 61.3 | 68.4 | ||
| ND5 | F13028 | CTGACTCCCCTCAGCCATAGA | 21 | 57.2 | 57.1 | 276 |
| R13303 | TGTGGTTGGTTGATGCCG | 18 | 53.3 | 55.6 |