PT - JOURNAL ARTICLE AU - Han, Yu AU - Yang, Yuming AU - Sun, Qiuyang AU - Li, Bin AU - Yue, Caixia AU - Liu, Yanlei AU - de la Fuente, Jesús M. AU - Cui, Daxiang TI - Dual-targeted lung cancer therapy <em>via</em> inhalation delivery of UCNP-siRNA-AS1411 nanocages AID - 10.20892/j.issn.2095-3941.2020.0416 DP - 2022 Jul 15 TA - Cancer Biology &amp; Medicine PG - 1047--1060 VI - 19 IP - 7 4099 - http://www.cancerbiomed.org/content/19/7/1047.short 4100 - http://www.cancerbiomed.org/content/19/7/1047.full SO - Cancer Biology &amp; Medicine2022 Jul 15; 19 AB - Objective: Although great progress has been made in the field of siRNA gene therapy, safe, efficient, and targeted delivery of siRNA are still major challenges in siRNA therapeutics.Methods: We developed an up-conversion nanoparticle-based nanocage system. This system protected the siRNA from being degraded by nucleases in organisms and selectively delivered the siRNAs to the tumor sites, due to modifications of targeted molecules on the surfaces of nanocages and local inhalation.Results: The siRNAs delivered by the up-conversion nanoparticle nanocages were protected from degradation in transit to the tumor sites, where they accumulated. Compared with the passive target and control groups, the up-conversion nanoparticles based on the nanocage system showed a tumor suppressive effect after approximately 3 weeks of treatment.Conclusions: The up-conversion nanoparticle nanocages efficiently delivered vascular endothelial growth factor siRNAs to tumor sites. Mice with lung tumors treated with tumors targeting up-conversion nanoparticle nanocages showed steady body weight changes, high tumor inhibition ratios, and longer survival times.