PT - JOURNAL ARTICLE AU - Yi Xie AU - Bing Wang AU - Liqing Du AU - Yan Wang AU - Chang Xu AU - Hong Zhang AU - Kaixue Wen AU - Qiang Liu AU - Takanori Katsube TI - ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines AID - 10.20892/j.issn.2095-3941.2020.0569 DP - 2022 Jul 15 TA - Cancer Biology & Medicine PG - 983--994 VI - 19 IP - 7 4099 - http://www.cancerbiomed.org/content/19/7/983.short 4100 - http://www.cancerbiomed.org/content/19/7/983.full SO - Cancer Biology & Medicine2022 Jul 15; 19 AB - Objective: The aim of the present study was to investigate the mechanisms responsible for the radiation-sensitizing effect of antennapedia proteins, ANTP-SMACN7, on lung cancer cells treated with accelerated carbon and Fe particle irradiation.Methods: The ANTP-SMACN7 fusion peptide was synthesized and linked to fluorescein isothiocyanate to determine its ability to penetrate cells. A549 and NCI-H460 cells, human non-small cell lung cancer (NSCLC) cell lines, were irradiated with X-ray or high linear energy transfer (LET) irradiation with or without ANTP-SMACN7 treatment. Cellular survival, apoptosis, and protein expression were studied by colony formation assays, flow cytometry, and western blot analyses, respectively.Results: ANTP-SMACN7 fusion proteins entered the cells and promoted A549 and NCI-H460 cell high LET irradiation radiosensitization. High LET irradiation was more efficient for clonogenic cell killing and the induction of apoptosis (P < 0.05). Treatment with ANTP-SMACN7 significantly reduced the A549 and NCI-H460 cell clone-forming percentages and increased apoptosis through inhibition of the X-linked inhibitor of apoptosis protein and the activation of caspase-3 and caspase-9.Conclusions: Regarding pharmaceutical radiosensitization, these findings provided a way to improve high-LET clinical radiotherapy for NSCLC patients.