PT  - JOURNAL ARTICLE
AU  - Dmitriy Sergeevich Kobyakov
AU  - Ashot Merudzhanovich Avdalyan
AU  - Aleksandr Fedorovich Lazarev
AU  - Elena Leonidovna Lushnikova
AU  - Lev Moiseevich Nepomnyashchikh
TI  - Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival
AID  - 10.7497/j.issn.2095-3941.2014.04.005
DP  - 2014 Dec 01
TA  - Cancer Biology and Medicine
PG  - 264--269
VI  - 11
IP  - 4
4099  - http://www.cancerbiomed.org/content/11/4/264.short
4100  - http://www.cancerbiomed.org/content/11/4/264.full
SO  - Cancer Biol Med2014 Dec 01; 11
AB  - Objective: To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC).Methods: A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohistochemical and AgNOR-staining methods.Results: The AgNOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM (tumor, node, and metastasis) system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological (AgNOR area in MIB-1-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC.Conclusion: The AgNOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.