RT Journal Article
SR Electronic
T1 Expression of the B-Cell Lymphoma/Leukemia 11A Gene in Malignant Hematological Cell Lines through Quantitative Reverse Transcription Polymerase Chain Reaction
JF Clinical Oncology and Cancer Research
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 242
OP 246
DO 10.1007/s11805-011-0588-2
VO 8
IS 4
A1 GAO, Yang-jun
A1 HE, Dong-mei
A1 CHEN, Shao-hua
A1 YAN, Xiao-juan
A1 HU, Xiao-mao
A1 LI, Yang-qiu
YR 2011
UL http://www.cancerbiomed.org/content/8/4/242.abstract
AB OBJECTIVE The B-cell lymphoma/leukemia 11A (BCL11A) gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).METHODS The relative expression level of BCL11A mRNA in malignant hematological cell lines was determined through qRT-PCR using SYBR Green I dye. Glyceraldehyde-3-phosphate dehydrogenase was used as the reference gene to confirm the relative expression level of BCL11A gene mRNA.RESULTS The relative expression level of BCL11A mRNA in cell lines from B-cell malignancies was significantly higher compared with that from acute myeloid leukemia (P < 0.05). Different cell lines with malignant B-cells exhibited a wide range of BCL11A expressions ranging from 27.37 to 93.38.CONCLUSION The overexpression of BCL11A gene mRNA in malignant B-cells might play a role in B-cell lymphoma/leukemia.