RT Journal Article SR Electronic T1 Comparative profiling of immune genes improves the prognoses of lower grade gliomas JF Cancer Biology & Medicine JO Cancer Biology & Medicine FD China Anti-Cancer Association SP 533 OP 550 DO 10.20892/j.issn.2095-3941.2021.0173 VO 19 IS 4 A1 Wang, Zhiliang A1 Cheng, Wen A1 Zhao, Zheng A1 Wang, Zheng A1 Zhang, Chuanbao A1 Li, Guanzhang A1 Wu, Anhua A1 Jiang, Tao YR 2022 UL http://www.cancerbiomed.org/content/19/4/533.abstract AB Objective: Lower grade gliomas (LGGs), classified as World Health Organization (WHO) grade II and grade III gliomas, comprise a heterogeneous group with a median survival time ranging from 4–13 years. Accurate prediction of the survival times of LGGs remains a major challenge in clinical practice.Methods: We reviewed the expression data of 865 LGG patients from 5 transcriptomics cohorts. The comparative profile of immune genes was analyzed for signature identification and validation. In-house RNAseq and microarray data from the Chinese Glioma Genome Atlas (CGGA) dataset were used as training and internal validation cohorts, respectively. The samples from The Cancer Genome Atlas (TCGA) and GSE16011 cohorts were used as external validation cohorts, and the real-time PCR of frozen LGG tissue samples (n = 36) were used for clinical validation.Results: A total of 2,214 immune genes were subjected to pairwise comparison to generate 2,449,791 immune-related gene pairs (IGPs). A total of 402 IGPs were identified with prognostic values for LGGs. The HOXA9-related and CRH-related scores facilitated identification of patients with different prognoses. An immune signature based on 10 IGPs was constructed to stratify patients into low and high risk groups, exhibiting different clinical outcomes. A nomogram, combining immune signature, 1p/19q status, and tumor grade, was able to predict the overall survival (OS) with c-indices of 0.85, 0.80, 0.80, 0.79, and 0.75 in the training, internal validation, external validation, and tissue sample cohorts, respectively.Conclusions: This study was the first to report a comparative profiling of immune genes in large LGG cohorts. A promising individualized immune signature was developed to estimate the survival time for LGG patients.The expression data were collected from the Chinese Glioma Genome Atlas (CGGA, http://www.cgga.org.cn/index.jsp), The Cancer Genome Atlas (TCGA, (http://cancergenome.nih.gov/) database, and the Gene Expression Omnibus database (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=gse16011).