RT Journal Article
SR Electronic
T1 Change and Significance of Mitochondrial DNA Copy Number in Esophageal Squamous Cell Carcinoma
JF Chinese Journal of Clinical Oncology
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 29
OP 32
DO 10.1007/s11805-007-00029-4
VO 4
IS 1
A1 Liu, Zongwen
A1 Zhao, Zhihua
A1 Zhao, Qiumin
A1 Li, Shenglei
A1 Gao, Dongling
A1 Pang, Xia
A1 Chen, Kuisheng
A1 Zhang, Yunhan
YR 2007
UL http://www.cancerbiomed.org/content/4/1/29.abstract
AB OBJECTIVE To compare the differences of mitochondrial DNA (mtDNA) copies among the tissues of esophageal squamous cell carcinoma (ESCC), para-neoplastic tissue and normal mucous membrane of the esophagus, and to study the relationship between the mtDNA and the occurrence and development of esophageal squamous cell carcinoma.METHODS The mtDNA copies of 42 specimens with the ESCC, paraneoplastic mucous tissue and normal mucous membrane of the esophagus were determined using real-time fluorescence quantitative PCR. The mtDNA was analyzed using agarose gel electrophoresis.RESULTS The mtDNA from all of the tissues (42/42) from the ESCC, para-neoplastic tissue and normal esophageal mucous membranes was analyzed, showing that there were an average mtDNA copy number of 27.1894×106 μg DNA, 9.4102×106 μg DNA and 5.9347×106 μg DNA, from the respective tissues. There were significant differences (F=27.83, P<0.05) in mtDNA copy number among the three. A positive band was shown at 403 bp after gel electrophoresis of the PCR products, and the lane where the ESCC mtDNA located was rather bright, which was in accordance with the result of the real-time PCR determination.CONCLUSION An increase in the mtDNA copy number is related to the occurrence and development of ESCC.