RT Journal Article
SR Electronic
T1 Efficacy of the new therapeutic approach in curing malignant neoplasms on the model of human glioblastoma
JF Cancer Biology and Medicine
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 910
OP 930
DO 10.20892/j.issn.2095-3941.2020.0511
VO 18
IS 3
A1 Evgeniya V. Dolgova
A1 Oleg M. Andrushkevich
A1 Polina E. Kisaretova
A1 Anastasia S. Proskurina
A1 Genrikh S. Ritter
A1 Tatyana D. Dubatolova
A1 Margarita V. Romanenko
A1 Oleg S. Taranov
A1 Yaroslav R. Efremov
A1 Evgeniy L. Zavyalov
A1 Alexandr V. Romaschenko
A1 Sergey V. Mishinov
A1 Svetlana S. Kirikovich
A1 Evgeniy V. Levites
A1 Ekaterina A. Potter
A1 Alexandr A. Ostanin
A1 Elena R. Chernykh
A1 Stanislav Yu. Roshchin
A1 Anatoliy V. Bervitskiy
A1 Galina I. Moysak
A1 Jamil A. Rzaev
A1 Sergey S. Bogachev
YR 2021
UL http://www.cancerbiomed.org/content/18/3/910.abstract
AB Objective: Glioma is a highly invasive tumor, frequently disposed in essential areas of the brain, which makes its surgical excision extremely difficult; meanwhile adjuvant therapy remains quite ineffective.Methods: In the current report, a new therapeutic approach in curing malignant neoplasms has been performed on the U87 human glioblastoma model. This approach, termed “Karanahan”, is aimed at the eradication of cancer stem cells (CSCs), which were recently shown to be capable of internalizing fragments of extracellular double-stranded DNA. After being internalized, these fragments interfere in the process of repairing interstrand cross-links caused by exposure to appropriate cytostatics, and such an interference results either in elimination of CSCs or in the loss of their tumorigenic potency. Implementation of the approach requires a scheduled administration of cytostatic and complex composite double-stranded DNA preparation.Results: U87 cells treated in vitro in accordance with the Karanahan approach completely lost their tumorigenicity and produced no grafts upon intracerebral transplantation into immunodeficient mice. In SCID mice with developed subcutaneous grafts, the treatment resulted in reliable slowing down of tumor growth rate (P < 0.05). In the experiment with intracerebral transplantation of U87 cells followed by surgical excision of the developed graft and subsequent therapeutic treatment, the Karanahan approach was shown to reliably slow down the tumor growth rate and increase the median survival of the mice twofold relative to the control.Conclusions: The effectiveness of the Karanahan approach has been demonstrated both in vitro and in vivo in treating developed subcutaneous grafts as well as orthotopic grafts after surgical excision of the tumor.