<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fontanella, Rosaria A.</style></author><author><style face="normal" font="default" size="100%">Sideri, Silvia</style></author><author><style face="normal" font="default" size="100%">Di Stefano, Chiara</style></author><author><style face="normal" font="default" size="100%">Catizone, Angiolina</style></author><author><style face="normal" font="default" size="100%">Di Agostino, Silvia</style></author><author><style face="normal" font="default" size="100%">Angelini, Daniela F.</style></author><author><style face="normal" font="default" size="100%">Guerrera, Gisella</style></author><author><style face="normal" font="default" size="100%">Battistini, Luca</style></author><author><style face="normal" font="default" size="100%">Battafarano, Giulia</style></author><author><style face="normal" font="default" size="100%">Del Fattore, Andrea</style></author><author><style face="normal" font="default" size="100%">Campese, Antonio Francesco</style></author><author><style face="normal" font="default" size="100%">Padula, Fabrizio</style></author><author><style face="normal" font="default" size="100%">De Cesaris, Paola</style></author><author><style face="normal" font="default" size="100%">Filippini, Antonio</style></author><author><style face="normal" font="default" size="100%">Riccioli, Anna</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">CD44v8-10 is a marker for malignant traits and a potential driver of bone metastasis in a subpopulation of prostate cancer cells</style></title><secondary-title><style face="normal" font="default" size="100%">Cancer Biology and Medicine</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2021-08-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">788-807</style></pages><doi><style  face="normal" font="default" size="100%">10.20892/j.issn.2095-3941.2020.0495</style></doi><volume><style face="normal" font="default" size="100%">18</style></volume><issue><style face="normal" font="default" size="100%">3</style></issue><abstract><style  face="normal" font="default" size="100%">Objective: Bone metastasis is a clinically important outcome of prostate carcinoma (PC). We focused on the phenotypic and functional characterization of a particularly aggressive phenotype within the androgen-independent bone metastasis-derived PC3 cell line. These cells, originated from the spontaneous conversion of a CD44-negative subpopulation, stably express the CD44v8-10 isoform (CD44v8-10pos) and display stem cell-like features and a marked invasive phenotype in vitro that is lost upon CD44v8-10 silencing.Methods: Flow cytometry, enzyme-linked immunoassay, immunofluorescence, and Western blot were used for phenotypic and immunologic characterization. Real-time quantitative polymerase chain reaction and functional assays were used to assess osteomimicry.Results: Analysis of epithelial–mesenchymal transition markers showed that CD44v8-10pos PC3 cells surprisingly display epithelial phenotype and can undergo osteomimicry, acquiring bone cell phenotypic and behavioral traits. Use of specific siRNA evidenced the ability of CD44v8-10 variant to confer osteomimetic features, hence the potential to form bone-specific metastasis. Moreover, the ability of tumors to activate immunosuppressive mechanisms which counteract effective immune responses is a sign of the aggressiveness of a tumor. Here we report that CD44v8-10pos cells express programmed death ligand 1, a negative regulator of anticancer immunity, and secrete exceptionally high amounts of interleukin-6, favoring osteoclastogenesis and immunosuppression in bone microenvironment. Notably, we identified a novel pathway activated by CD44v8-10, involving tafazzin (TAZ) and likely the Wnt/TAZ axis, known to play a role in upregulating osteomimetic genes.Conclusions: CD44v8-10 could represent a marker of a more aggressive bone metastatic PC population exerting a driver role in osteomimicry in bone. A novel link between TAZ and CD44v8-10 is also shown.</style></abstract></record></records></xml>