PT - JOURNAL ARTICLE AU - Dongli Yue AU - Shasha Liu AU - Tengfei Zhang AU - Yong Wang AU - Guohui Qin AU - Xinfeng Chen AU - Huanyu Zhang AU - Dong Wang AU - Lan Huang AU - Feng Wang AU - Liping Wang AU - Song Zhao AU - Yi Zhang TI - NEDD9 promotes cancer stemness by recruiting myeloid-derived suppressor cells <em>via</em> CXCL8 in esophageal squamous cell carcinoma AID - 10.20892/j.issn.2095-3941.2020.0290 DP - 2021 Aug 01 TA - Cancer Biology and Medicine PG - 705--720 VI - 18 IP - 3 4099 - http://www.cancerbiomed.org/content/18/3/705.short 4100 - http://www.cancerbiomed.org/content/18/3/705.full SO - Cancer Biol Med2021 Aug 01; 18 AB - Objective: Esophageal squamous cell carcinoma (ESCC) has high morbidity and mortality rates worldwide. Cancer stem cells (CSCs) may cause tumor initiation, metastasis, and recurrence and are also responsible for chemotherapy and radiotherapy failures. Myeloid-derived suppressor cells (MDSCs), in contrast, are known to be involved in mediating immunosuppression. Here, we aimed to investigate the mechanisms of interaction of CSCs and MDSCs in the tumor microenvironment.Methods: ESCC tissues and cell lines were evaluated. Neural precursor cell expressed, developmentally downregulated 9 (NEDD9) was knocked down and overexpressed by lentiviral transfection. Quantitative PCR, Western blot, immunohistochemistry, cell invasion, flow cytometry, cell sorting, multiplex chemokine profiling, and tumor growth analyses were performed.Results: Microarray analysis revealed 10 upregulated genes in esophageal CSCs. Only NEDD9 was upregulated in CSCs using the sphere-forming method. NEDD9 expression was correlated with tumor invasion (P = 0.0218), differentiation (P = 0.0153), and poor prognosis (P = 0.0373). Additionally, NEDD9 was required to maintain the stem-like phenotype. Screening of chemokine expression in ESCC cells with NEDD9 overexpression and knockdown showed that NEDD9 regulated C-X-C motif chemokine ligand 8 (CXCL8) expression via the ERK pathway. CXCL8 mediated the recruitment of MDSCs induced by NEDD9 in vitro and in vivo. MDSCs promoted the stemness of ESCC cells through NEDD9 via the Notch pathway.Conclusions: As a marker of ESCC, NEDD9 maintained the stemness of ESCC cells and regulated CXCL8 through the ERK pathway to recruit MDSCs into the tumor, suggesting NEDD9 as a therapeutic target and novel prognostic marker for ESCC.