<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sheng, Qingfeng</style></author><author><style face="normal" font="default" size="100%">Shi, Yurong</style></author><author><style face="normal" font="default" size="100%">Li, Qiang</style></author><author><style face="normal" font="default" size="100%">Hao, Jihui</style></author><author><style face="normal" font="default" size="100%">Niu, Ruifang</style></author><author><style face="normal" font="default" size="100%">Wei, Xiyin</style></author><author><style face="normal" font="default" size="100%">Yang, Yi</style></author><author><style face="normal" font="default" size="100%">Zhang, Lin</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Proteasome Inhibitors Sensitize Hepatocellular Carcinoma Cells to TRAIL</style></title><secondary-title><style face="normal" font="default" size="100%">Chinese Journal of Clinical Oncology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006-12-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">442-446</style></pages><volume><style face="normal" font="default" size="100%">3</style></volume><issue><style face="normal" font="default" size="100%">6</style></issue><abstract><style  face="normal" font="default" size="100%">OBJECTIVE To investigate the effect of proteasome inhibition on the sensitivity of carcinoma cells to TRAIL-inducing apoptosis, and to study the mechanism of the response.METHODS Human hepatocellular carcinoma cells, pretreated with the proteasome inhibitor, MG132, were cotreated with TRAIL. Western blot assays, immunoprecipitation and RT-PCR were performed to test the expression of the Bcl-2 family proteins and Bax mRNA.RESULTS We found that (i) proteasome inhibition sensitized the human hepatocellular carcinoma cells to TRAIL; and (ii) resulted in Bax accumulation before release of cytochrome C and induction of apoptosis. These results were associated with the ability of proteasome inhibitors to overcome Bcl-2-mediated antiapoptotic function; (iii) Bax is regulated by an ubiquitin/proteasome-dependent degradation pathway.CONCLUSION Proteasome inhibition sensitized hepatocellular carcinoma cells to TRAIL by the inhibition of the ubiquitin/proteasome-mediated Bax degradation pathway.</style></abstract></record></records></xml>