RT Journal Article
SR Electronic
T1 Expression of Tyrosine-kinase Receptors and Neurotrophins in Human Neuroblastomas
JF Chinese Journal of Clinical Oncology
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 852
OP 855
VO 2
IS 6
A1 Daojian Rong
A1 Haide Gao
A1 Jinhua Zhang
A1 Xinyuan Li
A1 Xu Li
A1 Jihong Zhang
YR 2005
UL http://www.cancerbiomed.org/content/2/6/852.abstract
AB OBJECTIVE The aim of this study was to investigate mRNA expression of tyrosine-kinase receptors (TRKs) and neurotrophins (NTs) in human neuroblastomas.METHODS Expression of TrkA, TrkB, TrkC and BDNF was quantitatively examined by reverse transcription-polymerase chain reaction (RT-PCR) in 27 cases of neuroblastomas.RESULTS The high and total rates of TrkA were expressed in significantly more tumors in a lower-stage group compared to a higher-stage group (P<0.05) and the high level of TrkA expression was correlated positively with the 2-year cumulative-survival rate of the patients (P<0.01). The high and total rates of TrkB were expressed in significantly more tumors in a higher-stage group compared to a lower-stage group (P<0.05). All 3 rates of BDNF expression between the 2 groups showed no statistical difference (P>0.05), but the co-expression ratio of TrkB and BDNF showed a remarkable significance in the higher-stage group more than in the lower-stage group (P<0.05). TrkC expression was usually accompanied by TrkA expression, but there was only a non-significant trend between TrkC expression and TrkA expression.CONCLUSION RT-PCR for mRNA expression of TRKs and NTs has important clinical significance relating to the tumor stage and outcome for patients with neuroblastomas.